摘要
以7个大果沙棘杂交组合及F1为试验材料,从120个随机引物中筛选出多态性丰富的28个引物,用于大果沙棘杂交F1代的RAPD分析,共扩增出601条谱带,其RAPD标记分离情况可分为符合孟德尔分离规律、偏离孟德尔分离规律和异常分离。结果表明,7个杂交组合F1中,72.30%的RAPD标记符合孟德尔遗传规律,23.65%的RAPD标记偏离孟德尔遗传规律,4.05%的RAPD标记属于异常分离。试验为探寻大果沙棘后代的遗传关系提供了可靠的数据,并为大果沙棘的引种、育种工作奠定了理论基础。
The experiment took seven hybrid combinations of big fruit sea-buckthorns and F1 as experimental materials, 28 primers with abundant polymorphism screened from 120 random primers were selected to analyze the segregation patterns of RAPD markers in F1, which obtained 601 amplified bands. According to the segregation patterns, RAPD markers were divided into three types： one was normal Mendelian inheritance with segregation ratio of nearly 1：1 or 3：1; the second one was deviated from Mendelian segregation ratio; the third one was the abnormal segregation in low frequency. The results showed in seven F1 cross combinations, 72.30% RAPD makers accorded with Mendelian inheritance; those of deviated from Mendelian segregation ratios were 23.65% averagely, and 4.05% RAPD makers showed the abnormal segregation ratio. The experiment provides reliable data to explore the genetic relationship of big fruit sea-buckthorn offspring, and lays the theoretic foundation for species introduction and breeding of big fruit sea-buckthorn.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2010年第1期43-46,共4页
Journal of Northeast Agricultural University
基金
东北农业大学创新项目(190205)
