摘要
目的证实结核性胸膜炎大鼠模型的建立方法;观察大鼠结核性胸膜炎的胸膜组织形态及超微结构特征,进一步探讨结核性胸膜炎的发病过程及胸液渗出与吸收不平衡的原因。方法健康雌性Wistar大鼠30只,随机分成2组,对照组6只,模型组24只,复制结核性胸膜炎动物模型;分别于建模后2、4、6、8、10、12、14、16、18、22、26及30d处死。观察大鼠胸腔内积液特征及胸膜结构变化,并进行病理学及超微病理学观察。结果模型组24只大鼠成功复制出结核性胸膜炎模型,形成胸腔积液及胸膜结核结节,后期胸膜肥厚粘连。透射电镜观察显示,早期间皮细胞微绒毛减少,细胞膜、细胞质、细胞器损伤,中期间皮细胞逐渐消失,纤维细胞与成纤维细胞增生,后期由增生的胶原纤维替代原有胸膜结构。结论接种卡介苗5周后向大鼠胸膜腔内注入标准人型结核菌株H37RV结核分枝杆菌悬液,可以建立结核性胸膜炎的动物模型。胸膜间皮细胞的损伤及丢失,影响了胸水的回吸收。胸膜类上皮细胞肉芽肿及纤维组织的增生可能导致淋巴管阻塞,减少了胸水的渗出,同时可能破坏了间皮细胞与淋巴孔之间形成的回吸收通路。
【Objective】To develop a rat model of tuberculosis pleurisy and to identify the rat tuberculosis pleurisy histo-structure and ultra-structure of different stages in order to identify the reason of chest fluid unbalanced effusion and absorption.【Methods】30 healthy female Wistar rats randomly divided into two groups,control group 6 rats,model group 24 rats,copy a rat model of tuberculosis pleurisy;sacrificed in different stage.The model group rats were killed in group on days 2,4,6,8,10,12,14,16,18,22,26 and 30.To observe the characters of the chest fluid and the change of the pleura structure,and observe the change of the pathology and ultra-pathology.【Results】To succeed copying the rat model of tuberculosis pleurisy in model group and to form chest fluid,tubercler and pachynsis pleurae.There were decreased of microvillus from mesothelial cell,damaged of cellular membrane,cytoplasm and cell organ,to reach the diminish of mesothelial cell,proliferation of fibrocyte and fibroblast,and proliferation of collagen fibers.【Conclusion】After injected BCG 5 weeks,injected tuberculosis bacilli H37RV suspension could establish a rat model of tuberculosis pleurisy.The damage of pleura mesothelial cell effects the absorb of chest fluid.The pleura epithelioid cell granuloma and the proliferation of fibrous tissue may block the lymph vessels,decrease the effusion of the chest fluid,and may damage the absorb way between the mesothelial cell and the ostium lymphaticum.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2009年第24期3721-3725,3730,共6页
China Journal of Modern Medicine
关键词
结核
胸膜炎
病理学
超微病理学
tuberculosis
pleurisy
pathological
ultrastructural pathology
