摘要
目的研究黄皮酰胺对糖尿病大鼠血糖、糖化血红蛋白及海马胶质纤维酸性蛋白(GFAP)mRNA表达的影响。方法利用链脲佐菌素制备大鼠糖尿病模型,将糖尿病大鼠随机分为模型组、胰岛素治疗组(10 U.kg-1胰岛素皮下注射)、黄皮酰胺高剂量治疗组(50 mg.kg-1黄皮酰胺灌胃给药)和黄皮酰胺低剂量治疗组(25 mg.kg-1黄皮酰胺灌胃给药),另取大鼠腹腔注射pH 4.4柠檬酸缓冲液作为对照组,每组15只。给药3个月后,检测比较各组大鼠血糖和糖化血红蛋白的不同;通过反转录聚合酶链式反应(RT-PCR)技术观察海马GFAP mRNA的表达。结果给药3个月后,与对照组和胰岛素治疗组相比,模型组和黄皮酰胺高、低剂量组大鼠的血糖和糖化血红蛋白值明显升高(P<0.01);而黄皮酰胺组大鼠的血糖和糖化血红蛋白值与模型组相比差异无统计学意义(P>0.05);RT-PCR结果显示,模型组大鼠海马的GFAP mRNA表达水平明显增加(P<0.01);而黄皮酰胺高、低剂量组大鼠海马的GFAP mRNA的表达水平明显降低(P<0.01)。结论黄皮酰胺可能通过抑制海马GFAP mRNA的表达对糖尿病大鼠起到保护作用,但没有降低血糖和糖化血红蛋白的作用。
Objective To investigate the influence of elausenamide on blood sugar, glyeosylated hemoglobin and hippoeampal glial fibrillary acidic protein （GFAP） mRNA expression in diabetic rats. Methods The diabetic rats were randomly divided into model group, insulin treatment group （10 U·kg^-1 insulin, hypodermic injection）, high dose clausenamide group （50 mg·kg^-1 elausenamide, intragastrie administration） and low dose clausenamide group （25 mg·kg^-1 clausenamide, intragastrie administration） ,fifteen rats in each group. Fifteen rats in control group were treated with citrate buffer solution by intraperitoneal injection. The diabetic rats model produced by injection streptozotocin were treated by clausenamide. After 3 months,blood sugar and glycosylated hemoglobin were observed, RT-PCR was used to detect mRNA expression of GFAP in hippocampus of diabetic rats. Results Compared with control group, the levels of blood sugar and glycosylated hemoglobin in model group increased obviously（ P 〈0.01 ） ,but there was no statistical difference in the levels of blood sugar and glycosylated hemoglobin between the model group and clansenamide group （P 〉 0.05 ）. The results of RT-PCR showed that the expression of GFAP mRNA in hippoeampus of model group was higher than that of control group（ P 〈 0.01 ）. The expressions of GFAP mRNA of clausenamide groups and insulin treatment group were lower than that of model group （ P 〈 0.01 ）. Conclusion Clausenamide can significantly inhibit GFAP mRNA expression in hippocampus ,which may account for the protective effect. But clausenamide can not degrade the levels of blood sugar and glyeosylated hemoglobin.
出处
《新乡医学院学报》
CAS
2009年第6期562-565,共4页
Journal of Xinxiang Medical University
关键词
黄皮酰胺
糖尿病
血糖
糖化血红蛋白
海马
胶质纤维酸性蛋白
clausenmaide
diabetes mellitus
blood sugar
glycosylated hemoglobin
hippocampns
glial fibrillary acid protein
