摘要
目的研究大鼠脑出血(ICH)后血肿周围白细胞介素10(IL-10)、白细胞介素18(IL-18)的表达。方法将65只SD大鼠随机分为正常对照组、假手术组和ICH组,应用肝素化Ⅶ型胶原酶建立大鼠ICH模型,免疫组化检测脑组织血肿周围IL-10、IL-18的表达。结果(1)ICH组大鼠12h脑组织IL-10表达即开始上升,7d明显升高,14d达高峰,各时间点与正常对照组和假手术组相比均有统计学意义(P<0.05);(2)ICH组大鼠12h脑组织IL-18表达即开始上升,7d达高峰,各时间点与正常对照组和假手术组比较均有统计学意义(P<0.01);(3)ICH组IL-18与IL-10呈显著正相关(r=0.909,P<0.01)。结论ICH后血肿周围存在着IL-10、IL-18介导的炎性反应,两者的相互作用可能接近于一个相对动态的平衡点,有利于疾病的恢复。
Objective To study the IL-10 and IL-18 expression around the hematoma after experimental intracerebral hemorrhage（ICH） in rats,and to discuss their relationship.Methods 65 rats were randomly divided into three groups： normal control group,sham operated group and ICH group.The ICH model in rats was established by heparinized Ⅶ-type collagenase,and the expression of IL-10,IL-18 in cerebral tissues at different groups were determined by immuno-histochemistry.Results In ICH group,the expression of IL-10 in cerebral tissues at 12 hours began to heighten,increased obviously at 7 days,and reached to the peak at 14 days.There were significant differences with normal control group and sham operated group at different time points（P0.05）.In ICH group,the expression of IL-18 in cerebral tissues at 12 hours began to heighten,reached to the peak at 7 days,and the differences at different time points statistic between normal control group and sham operated group were significant（P0.01）.In ICH group,there were positive correlation of IL-10 and IL-18（r=0.909,P0.01）.Conclusion IL-10 and IL-18 takes part in the inflammatory process around the hematoma in ICH,their interaction may get close a relatively dynamic point of balance.
出处
《宁夏医学杂志》
CAS
2009年第11期971-973,F0002,共4页
Ningxia Medical Journal
基金
宁夏自然科学基金资助项目(NZ08144)
