摘要
目的研究大鼠双侧耳蜗毁损后下丘核(inferior colliculus,IC)生长相关蛋白-43(growth-associat-ed protein43,GAP-43)表达水平的变化。方法成年健康SD大鼠35只,随机分为假手术组(对照组)和耳蜗毁损后3、7、14、21、28、90天组,每组各5只动物。应用耳蜗毁损手术法制备大鼠双侧耳蜗毁损模型,采用免疫组织化学方法检测耳蜗毁损后大鼠下丘核GAP-43的表达。结果各组大鼠双侧耳蜗毁损后下丘核内GAP-43均有表达,耳蜗毁损各组表达均高于对照组,毁损后3、7、14天GAP-43表达持续上升,14天时达高峰,21、28天逐渐下降,到毁损后90天GAP-43水平略高于正常组;除90天组外,耳蜗毁损各组的GAP-43表达水平与对照组比较,差异均有统计学意义(P<0.05)。各组两耳间差异均无统计学意义。结论双侧耳蜗去传入损伤后,下丘核内GAP-43表达14天时达高峰,90天时接近正常水平,可能反映了下丘核内神经元的轴突生长及突触重塑情况。
Objective To explore the expression of growth associated protein-43（GAP-43） in the inferior colliculus in rats following bilateral cochlear ablation.Methods 35 healthy adult SD rats were randomly divided into 7 groups：3,7,14,21,28,90 days following bilateral cochlear ablation and the sham operation group as the control group.The immunohistochemistry was used to observe the expression of GAP-43 after bilateral cochlear ablation in each group.Results GAP-43 was expressed in each group. The GAP-43 expression in bilateral cochlear ablation group were higher than that in the control group. The expression increased at 3 days ,followed by a constant increase at 7 to 14 days with the peak on day 14. On day 21 the GAP-43 expression began to decrease. The mean gray level of GAP-43 in all the staining of groups had statistic significance compared to the control group except the group of day 90. However ,in all the 7 groups,the mean gray level between the left and right ears had no statistic significance.Conclusion The change of GAP-43 expression in the inferior colliculus following bilateral cochlear ablation reflects synaptic plasticity in inferior colliculus the neurons.
出处
《听力学及言语疾病杂志》
CAS
CSCD
北大核心
2010年第1期48-51,共4页
Journal of Audiology and Speech Pathology
基金
国家自然基金面上项目(30772397)资助
