摘要
目的探讨血管内皮细胞生长因子（ＶＥＧＦ）和血管生成与胃癌发展的关系。方法应用免疫组织化学和原位分子杂交技术，检测５６例人胃癌组织ＶＥＧＦ蛋白表达和微血管密度（ＭＶＤ）及部分胃癌ＶＥＧＦｍＲＮＡ表达，分析ＶＥＧＦ和ＭＶＤ、及其与胃癌组织学分型、浸润深度、生长方式、淋巴结转移、远处转移和预后的关系。结果ＶＥＧＦ阳性者ＭＶＤ值显著高于阴性者（Ｐ＜００１），ＶＥＧＦ表达和ＭＶＤ与胃癌浸润深度（Ｐ＜００１）、淋巴结转移（Ｐ＜００５）和远处转移（Ｐ＜０．０５）密切相关，而与组织学分型和生长方式无关（Ｐ＞００５）；ＶＥＧＦ表达阳性或ＭＶＤ≥４３的胃癌患者５年生存率较低；ＶＥＧＦｍＲＮＡ表达与ＶＥＧＦ蛋白表达具有一致性，但其分布不同。结论ＶＥＧＦ与胃癌的血管生成密切相关，对胃癌的生长和浸润转移有促进作用。
Objective To investigate the relationship of vascular endothelial growth factor (VEGF) and angiogenesis to the progression of gastric carcinoma (GC). Methods VEGF protein expression and microvessel density (MVD) in 56 cases of human GC and VEGF mRNA expression in some cases were examined by use of immunohistochemical staining and in situ hybridization technique respectively. MVD and VEGF expression were analysed together with their relation to histologic types, depth of invasion, growth pattern, lymph node metastasis, distant metastasis and relapsing rate of GC. Results MVD was significantly higher in VEGFpositive GC than in VEGFnegative GC (P<001). MVD and VEGF positively correlated with depth of invasion (P<0.01), lymph node metastasis (P<0.05) and distant metastasis (P<0.05), but their relation to the histologic types and growth pattern of GC was not significant. The 5 year survival rate was significantly lower in GC patients with positive expression of VEGF or with MVD≥43. The expression of VEGF mRNA was consistent with that of VEGF protein, but the distribution is different. Conclusion VEGF is highly related to angiogenesis of GC and promotes growth, invasion and metastasis of GC. VEGF expression or MVD is one of the predictors for the biological behavior of GC.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
1998年第4期278-281,共4页
Chinese Journal of Pathology
关键词
胃肿瘤
内皮生长因子
新生血管化
肿瘤转移
Stomach neoplasms Endothelial growth factors Neovascularization Neoplasm metastasis
