摘要
目的:探讨石上柏对人鼻咽癌TW03细胞的生长抑制作用及其可能的机制。方法:采用MTT法检测TW03细胞的生长抑制率,流式细胞仪检测TW03细胞周期改变,免疫组化法检测TW03细胞中Bcl-2和Bax的表达。结果:随着石上柏质量浓度的增加和作用时间的延长,TW03细胞生长抑制率显著升高(P<0.05)。随着石上柏质量浓度的增加,S期TW03细胞所占比例逐渐上升,而G_0/G_1期和G_2/M期所占比例逐渐下降,同时Bc1-2表达下调,而Bax表达上调。结论:石上柏能通过诱导人鼻咽癌TW03细胞周期阻滞于S期,其机制可能与下调Bcl-2表达和上调Bax的表达有关。
Objective：To study the inhibition and mechanism of Selaginella doederleinii（SLG） on the growth of nasopharyngeal carcinoma（NPC） TW03 cells in vitro.Methods：Cell suppression rates were measured by MTT assay,cell cycles were measured by flow cytometry（FCM） and expressions of Bcl-2 and Bax protein were detected by immunohistochemistry.Results：Inhibition on the growth of NPC cell was intensed by SLG in a dose and time dependent manner in vitro（P〈0.05）.The ratio of S phase cells increased and the ratios of G0/G1,and G2/M phase cells decreased,with the addition of SLG.Meanwhile,the expression of Bcl-2 protein was down-regulated and Bax protein was up-regulated.Conclusion：SLG could inhibit the growth of human NPC TW03 cells by directing cell cycle arrested at S phase,down-regulating Bcl-2 protein expression and up-regulating Bax protein expression.
出处
《中药材》
CAS
CSCD
北大核心
2009年第12期1864-1867,共4页
Journal of Chinese Medicinal Materials
基金
广西科技厅攻关课题(桂科攻032202421A)
