摘要
目的:探讨不同浓度的二苯乙烯苷(TSG)对1-甲基-4苯基吡啶离子(MPP+)诱导的PC12细胞损伤的保护作用.方法:MTT比色试验检测细胞活性;Hoechst33258染色观察凋亡细胞核形态;流式细胞仪(FCM)检测细胞凋亡比例;TUNEL酶标记法检测凋亡细胞断裂的DNA片段.结果:1,5,10μmol/LTSG对MPP+诱导的PC12细胞损伤具有一定的保护作用.与MPP+组相比,1,5,10μmol/LTSG预处理组细胞活力分别上升为(57.8±1.2)%(P<0.05),(74.3±2.7)%(P<0.05),(86.8±2.0)%(P<0.05).Hoechest33258染色发现MPP+组较多胞核出现固缩凝集,而TSG处理组预处理组则分别减少为(31.6±2.3)%,(22.4±1.8)%,(13.4±1.1)%(P<0.05).FCM检测也提示TSG预处理可降低细胞凋亡百分率.TUNEL染色显示MPP+组TUNEL阳性细胞(35.3±1.2)%增加,而TSG预处理组TUNEL阳性细胞显著降低为(25.3±1.2)%,(17.3±0.9)%,(11.7±0.9)%.结论:TSG可减轻MPP+诱导的PC12细胞损伤和凋亡.
AIM：To study the protective effect of 2,3,5,4,-tetrahydroxystilbene-2-O-β-D-glucoside（TSG） on the injury of PC12 cells induced by 1-methy-4-phenylpyridinium（MPP＋）.METHODS：Using MPP＋ induced PC12 cells damage as the model of Parkinson＇s disease in vitro,the protevtive effect of TSG was explored.The cytotoxicity of MPP＋ was measured by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide（MTT） assay,the morphological change of the nuclear was detected by Hoechest 33258 staining,the apoptotic percentage of PC12 cells was detected by flow cytometry（FCM） and the fragmentation of DNA was assessed with TUNEL staining.RESULTS：1,5,10 μmol/L TSG respectively played a protective role against MPP＋ induced PC12 cells injury.Compared with MPP＋ treated cells,after exposure with 1,5 and 10 μmol/L TSG,cell viability was increased to（57.8±1.2）%,（74.3±2.7）%,（86.8±2.0）%,respectively.Hoechest 33258 staining demonstrated that more cells were characterized by nuclear condensation in MPP＋ group,while in the group of TSG,the rate was decreased to（31.6±2.3）%,（22.4±1.8）% and（13.4±1.1）%,respectively.FCM analysis indicated that apoptotic ratio was decreased to 27.2%,19.1% and 13.3% after TSG（1,5 and 10 μmol/L） pretreatment,while in control and MPP＋ group,the ratio was 1.5% and 35.6%.TUNEL staining indicated that TUNEL-positive cells were increased in the MPP＋ treated group and markedly reduced to（25.3±1.2）%,（17.3±0.9）% and（11.7±0.9）% respectively after pretreated with TSG.CONCLUSION：The data indacates that TSG has a protective effect on the injury of PC12 cells induced by MPP＋.
出处
《第四军医大学学报》
北大核心
2009年第24期2910-2913,共4页
Journal of the Fourth Military Medical University
基金
国家自然科学基金(30772743)
