摘要
目的:建立大鼠PDLLA/FK506(外消旋聚乳酸/FK506)复合套管坐骨神经再生模型,观察大鼠坐骨神经再生过程中行为学、痛阈以及脊髓背角c-fos表达的改变,研究大鼠PDLLA/FK506复合套管外周神经再生模型中神经病理痛的变化.方法:雄性Wistar大鼠40只,体质量180～200g,随机分成2组,每组20只,A组为坐骨神经损伤PDLLA/FK506套管修复组;B组为假手术组.术后27d起检测行为学、机械刺激阈值、热缩足反射时间及c-fos表达计数.结果:A组术后27d后机械痛阈和热缩足反射潜伏期与B组相差明显,有统计学意义(P<0.05).A组术侧L4～5脊髓背角与B组相比较,背角浅层c-fos计数增多,有统计学意义(P<0.05).A组组内c-fos、机械痛阈和热缩足反射潜伏期45d与27d比较均有统计学意义(P<0.05).结论:大鼠PDLLA/FK506复合套管外周神经再生模型中神经再生过程中诱发脊髓背角浅层c-fos表达,同时出现再生神经支配区域的神经病理痛和疼痛相关行为.
AIM： By observing pain threshold and changes of c-los expression in the dorsal horn in PDLIA/FKS06 compound eondit peripheral nerve regeneration model to investigate regeration nerve ＇s neuropathic pain. METHODS： Forty male Wistar rats weighing 180-200 g were randomly divided into 2 groups： PDLLA/FKS06 compound eondit regeneration model group （group A） and sham-operation group（group B）. The fight sciatic nerve of group A underwent transection and then repaired with PDLLA/ FKS06 compound condit while 10 mm nerve defect was kept. The right sciatic nerve was just exposed in B group without injury. Behavioral response, pain threshold and thermal withdrawal latency were assessed on 27,30,33,36,39,42 and 45 d after operation. C-fos expression was exmianed immunohistochemistry on 27,33, 39 and 45 d after operation. RESULTS： The cumulative pain scores of group A were higher than those of group B at every time point（P 〈0.05 ）, while no significant difference existed between each time point in group A. The count of neurons with positive Fos protein in eornu dorsale medullae spinalis IA-5 of group A was higher than that of group B （ P 〈 0.05 ）, and significant differ- ence was found between the c-fos expression level on 27 d com- pared with that of 45 d after operation in group A （ P 〈 0.05 ）. As for pain threshold and TWL , more strick contrast between the value of 27 and 45 d after opration exist in group A compared with that in group B（ P 〈 0.05 ）, and significant difference was found between the level on 27 d compared with that of 45 d after opera- tion in group A （ P 〈 0.05 ）. CONCLUSION ： The regeneration of injured peripheral nerve may induce the c-los expression and also hvperDathia and changes in pain-related behavior in PDLLA/FK506 compound condit peripheral nerve regeneration model.
出处
《第四军医大学学报》
北大核心
2009年第24期2940-2943,共4页
Journal of the Fourth Military Medical University
基金
湖北省卫生厅科研基金(JX2B45)
