摘要
目的观察3周尾部悬吊模拟失重对大鼠不同部位骨的骨密度(BMD)的影响,并探讨中药复方对其干预作用。方法2月龄雄性Wistar大鼠,按体重随机分为3组:空白组、模型组、中药组。中药组全程给予中药复方(含刺五加、熟地黄、牛膝、牡蛎等)水煎剂灌胃,空白组和模型组则仅给予与中药组等量、等形式的牡蛎(均以等量醋酸水解);自第2周始,模型组和中药组均以尾部悬吊模拟失重3周。第4周末大鼠被处死后,分别测量其7个不同部位骨的BMD,其中头盖骨和第二胸椎用浮力法测量,第四腰椎、肱骨、股骨、桡尺骨和胫骨用双能X线骨密度仪测量。结果与空白组相比,模型组头盖骨BMD显著增加(P<0.05),胸椎、桡尺骨、肱骨无变化(P>0.05),腰椎、股骨、胫骨BMD显著下降(P<0.05,P<0.01);与模型组比较,中药组腰椎、股骨、肱骨BMD显著提高(P<0.05,P<0.01),同时胫骨BMD有提高趋势,而头盖骨、桡尺骨、胸椎无变化(P>0.05)。结论3周尾吊模拟失重大鼠全身BMD变化呈现特定趋势,头部BMD提高,上肢及第二胸椎BMD保持不变,第四腰椎及后肢BMD下降;中药复方对缓解失重骨质疏松、纠正失重性骨钙再分布有积极意义。
Objective To investigate the effect of hindlimb unloaded 3 weeks on bone mineral density of different parts in simulated weightlessness rats, and the effect of Chinese herbal prescriptions on them. Methods 2-month-old male Wistar rats were randomly divided into 3 groups., control group （CON）, hindlimb unloading group （HU）, HU＋Chinese herbal prescription group （HUC）. During the whole experiment （4 weeks）, HUC were given Chinese herbal prescriptions （contains Radix Acanthopanacis Senticosl, Radix Rehmanniae Praeparata, Radix Achyranthis Bidentatae, Concha Ostreae, etc）, while CON and HU were given equal measures Concha Ostreae prepared by acetic acid. After 1 week of adaption period, weightlessness was simulated by tail suspension in HU and HUC for three weeks. Rats were executed at the end of the fourth week and bone mineral density of 7 differernt parts were determined. BMD of skull bone and second thoracic vertebra were examined by buoyancy method, and fourth lumbar vertebra, humerus, femur, radio-ulna and tibia were examined by bone densitometer. Results Compared with CON, BMD of skull of HU increased significantly （P〈0.05）, meanwhile, thoracic vertebra, radio-ulna and humerus were similar （P〉0.05）, but BMD of lumbar vertebra, femur and tibia were decreased notably （P〈0.05, P〈0.01）. Compared with HU, BMD of lumbar bertebra, femur and humerus of HUC increased significantly （P〈0.05）, a positive trend of tibia were shown in HUC, and BMD of skull bone, radio-ulna and thoracic vertebra were similar （P〉0.05）. Conclusions The change of hindlimb unloaded 3 weeks on systemic BMD presented certain trend. BMD of skull bone increased, little change on upper limbs and second thoracic vertebra, and fourth lumbar vertebra and hind limbs reduced. Chinese herbal prescriptions had the function to relieve weightlessness osteoporosis, and had positive effect on calcium distribution with weightlessness.
出处
《中国中医药信息杂志》
CAS
CSCD
2010年第1期28-30,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家自然科学基金(30500663)
2006年新世纪优秀人才支持计划(NCET-06-0124)
2006年北京市科技新星(A)类计划(2006A49)
北京中医药大学国家基础科学人才培养基金(20070110)
关键词
中药
模拟失重
骨密度
大鼠
Chinese herbal prescriptions
simulated weightlessness
bone mineral density
rat
