摘要
采用响应曲面法对乳牛肝菌发酵培养基及发酵条件进行优化,以麦芽糖、蛋白胨和装液量3个因素为变量,分析乳牛肝菌液态发酵产菌丝体的最佳条件。实验结果表明,乳牛肝菌发酵的关键因子值分别为麦芽糖33.03g/L,蛋白胨6.95g/L,装液量为102.21mL/250mL时,可得到最大菌丝体生物量23.44g/L(干重)。
In order to obtain the maximal mycelia hiomass of Suillus bovinus, response surface methodology was involved in this paper to optimize the culture medium and fermentation conditions. The critical variables selected for this experiment were maltose, protein peptone and broth content. The results showed that the maximal dry mycelia biomass could be achieved at 23.44g/L when the key fermentation factors like maltose, protein peptone and broth content were 33.03g/L, 6, 95g/L and 102. 21mL, respectively.
出处
《生物学杂志》
CAS
CSCD
2010年第1期5-8,共4页
Journal of Biology
基金
国家自然科学基金资助项目(20576002
20976138)
上海市自然科学基金资助项目(09ZR1434500)
关键词
乳牛肝菌
响应曲面法
液态发酵
优化
菌丝体生物量
Suillus bovinus
response surface methodology
submerged fermentation
optimum
mycelia biomass
