摘要
目的探讨基质细胞衍生因子-1(SDF-1)和CXCR4在小鼠角膜碱烧伤新生血管形成中的作用。方法应用在角膜中央放置NaOH滤纸片的方法构建C57/BL小鼠角膜碱烧伤角膜新生血管动物模型,通过免疫组织化学、RT-PCR、Western blot法检测碱烧伤后不同时间点角膜组织中SDF-1和CXCR4 mRNA及蛋白的表达情况。结果免疫组织化学检测显示,正常小鼠角膜基质层无明显SDF-1的阳性表达,CXCR4仅在角膜上皮层呈弱阳性表达。RT-PCR检测显示,与正常对照组相比,碱烧伤后各时间点SDF-1和CXCR4 mRNA表达均明显升高(P<0.05);Western blot检测显示SDF-1和CXCR4蛋白的表达也可见相似的变化趋势(P<0.05)。SDF-1和CXCR4在角膜中的表达于第7天达高峰,第14天开始下降,但仍高于正常。结论SDF-1/CXCR4在碱烧伤后小鼠角膜组织中的表达增加,在角膜碱烧伤的炎症和新生血管的形成和发展过程中可能发挥重要作用。
Background Recent research showed that stromal cell-derived factor-1 （SDF-1） plays critical role in mediating inflammation, metastasis of tumour and neovascularization of tumour. However, There is still no report about the research of the effects of SDF-1 on alkali-burn-induced corneal neovaseularization （ CNV）. Objective The aim of this study attempts to investigate the role of SDF-1 and chemokine receptor CXCR4 in alkali-induced corneal neovascularization in mice. Methods Alkali-induced-corneal neovascularization animal models were constructed of in 15 eyes of 15 clean C57/BL mice by placing the filter paper with 1 mol/L NaOH to the central cornea for 10 seconds. The animals were sacrificed and specimens of cornea were obtained in 3,7,14 days after alkali burn of cornea. The expression of SDF-1/CXCR4 protein in the corneal tissues was detected by immunohistochemistry and Western blot,and expression of SDF-1/CXCR4 mRNA was detected by reverse transcription PCR （RT-PCR）. Results SDF-1 was absent expressed and CXCR4 was faintly stained in normal cornea. The expression level of SDF-1 and CXCR4 was significantly enhanced in different time points after alkali burn in comparison with normal control mice by RT-PCR（P 〈0.05） ,and the same trend was seen in the expression level of SDF-1 and CXCR4 proteins by Western blot （P 〈 0.05 ）. The expression level of SDF-1 and CXCR4 in cornea peaked in the seventh day and began to decline in the fourteenth day but was still higher than normal level. Conclusion The study indicates that SDF-1/CXCR4 plays an important role in the formation of corneal new vessel in alkali-burn-induced animal model.
出处
《眼科研究》
CSCD
北大核心
2010年第2期135-139,共5页
Chinese Ophthalmic Research
