摘要
目的探讨芦丁提取与纯化的新工艺研究,提高芦丁的提取率与纯度。方法用乙醇作为溶剂,从槐米中提取芦丁,提取液浓缩至无醇味静置,待芦丁遇冷结晶后,离心取下层沉淀,用冷水继续洗涤结晶。结果槐米中芦丁提取最佳工艺为15倍70%乙醇提取3次,每次1h,最佳纯化工艺为:提取液浓缩至无醇味静置形成结晶后,离心,20倍水洗涤3次,芦丁纯度可达89.82%。结论本实验建立了一种提取、纯化芦丁的新工艺,该工艺相对简便易行,经济实用,节约生产成本,提高生产效率。
OBJECTIVE In order to improve the extraction efficiency and fineness of Rutin, we did some study on new methods of extraction and purification. METHODS First, extracted Rutin from Flos Sophorae by using ethanol as the resolvent, then evaporated the ethanol and got the crystal by centrifugation. Second, purificated the crystal by cold water. RESULTS Extration Rutin was 15 times ethanol of 70 % concentration, 1 hour everytime and 3 times.The optimization method of Rutin purification was the way that washed by cold water 3 times, and using 20 times cold water everytime. After the third-time wash, Rutin could attain the purification of 84.26% . CONCLUSION We established new methods of extraction and purification. The methods were simple, convenient, managing and high efficiency.
出处
《海峡药学》
2010年第1期49-51,共3页
Strait Pharmaceutical Journal
关键词
芦丁
纯化
Rutin
Purification
