摘要
从１９９７年在浙江省建德市发生的一种新的以腺胃肿大为特症的鸡传染病病例中收集的腺胃组织（ＺＪ９７１）为鸡胚接种材料，感染鸡胚第１代可达３３．３％的死亡率，第３代达７５％的死亡率并出现规律性死亡，自第６代开始死亡率达１００％．鸡胚尿囊液经１％胰酶处理后可凝集鸡和鼠的红细胞，电镜观察可见直径９０～１９０ｎｍ的带囊膜和突起的冠状病毒粒子，中和试验结果显示低滴度的ＺＪ９７１可被ＩＢＶ－Ｈ５２和Ｔ株血清中和，ＺＪ９７１对酸、碱、热、乙醚和氯仿敏感，对反复冻融不敏感．这表明，从腺胃病变型鸡群中分离的ＺＪ９７１毒株是冠状病毒科的一个成员．
Outbreaks of a new chicken′s infectious disease occurred in some chicken′s farms in Jiande city in Zhejiang province since 1997, was characterised by the severe swell of proventriculus. Mortality of chicken′s embryos inoculated with suspensions of proventriculus of the sick died chickens (ZJ 971 isolates) was respectively 33.3%, 75%, 100% after passing 1 time, 3 times and 6 times. Chicken and mouse erythrocytes were respectively agglutinated by embryo urine of the infected chicken′s embryo digested by 1% trypsin. Electron microscopic examinations of negatively stained preparations revealed that the diameter of virions (ZJ 971 isolates) covered with an envelope connected with the pedunculated projections was about 90-190 nm. Lower titer of ZJ 971 isolates was respectively neutralized by hyperimmune antiserum against infectious bronchitis virus H 52 and T strain. ZJ 971 isolates was sensitive to heat , acid , base , ether , chloroform , and insensitive to repeated freeze melt. These results shows that virus(ZJ 971 isolates) isolated from the sick flocks characterized with the swell of the proventriculus was a member of coronaviridae.
