期刊文献+

一株产脂肽类生物表面活性剂菌株的分离及代谢产物分析 被引量:10

Isolation and Metabolite Analysis of A Strain Producing Lipopeptide Biosurfactant
下载PDF
导出
摘要 采用多次富集培养、血平板筛选方法,从新疆克拉玛依油田油水样中分离得到产生物表面活性剂菌株L1。该菌株与已培养的土壤芽孢杆菌(Brevibacillus agri)的16S rDNA序列同源性达到99%;其代谢产物具有降低表面张力的作用,可以将发酵液表面张力从最初的69.56 mN.m-1降到29.36 mN.m-1;菌株代谢产物经薄层层析分析初步鉴定为脂肽类生物表面活性剂,红外光谱定性该生物表面活性剂属于环脂肽类表面活性剂。 After multiple rounds of enrichment,blood-plate treatment, strain L1 producing lipopeptide biosurfactant was isolated from the oil water sample of the Kelamayi Oilfield. Phylogenetic rDNA gene sequence indicated that strain L1 was closest to Brevibacillus agri with 99 % tension of cultured fluid of strain L1 reduced from 69.56 mN · m^-1 to 29.36 mN · m^- 1 , identified preliminarily as lipopeptide biosurfactant by thin layer chromatography and as surfactant by FTIR spectroscopy. analysis based on 16S similarity. The surface and the metabolite was a cyclic lipopetide biosurfactant by FTIR spectroscopy.
出处 《化学与生物工程》 CAS 2010年第2期46-49,共4页 Chemistry & Bioengineering
基金 国家863计划资助项目(2008AA06Z204)
关键词 脂肽类生物表面活性剂 代谢产物 分离 血平板筛选法 16SrDNA 土壤芽孢杆菌 lipopeptide biosurfactant metabolite isolation blood-plate treatment 16S rDNA Brevibacillus agri
  • 相关文献

参考文献14

  • 1Soumen Mukherjee , Palashpriya Das , Ramkrishna Sen . Rapid quantification of a microbial surfactant by a simple turbidometric method[J]. Journal of Microbiological Methods, 2009,76 ( 1 ) : 38-42.
  • 2佘跃惠,夏晶晶,黄金凤,王正良,张凡,王文军,王洪志.大庆油田聚驱后油藏驱油本源菌研究[J].油田化学,2009,26(1):98-101. 被引量:11
  • 3Kosaric N, Cairns W L. Biosurfactants and Biotechnology[M]. New York: Marcel Dekker Inc, 1987:21-45.
  • 4Peypoux F, Bonmatin J M, Wallach J. Recent trends in the biochemistry of surfactin[J]. Appl Mierobiol Bioteeh, 1999, 51(5) : 553-563.
  • 5Tulin E E, Amaki Y, Nagasawa T, et al. A Bacillus stearotherrnophilus esterase produced by a recombinant Bacillus brevis stabilized by sulfhydryl compounds[J]. Biosci Bioteehnol Bioehem, 1993, 57(5): 856-857.
  • 6Batista S B, Mounteer A H, Amorim F R,et al. Isolation and characterization of biosurfactant/bioemulsifier-producing bacteria from petroleum contaminated sites[J]. Bioresour Technol, 2006, 97(6) :868 875.
  • 7Costa S G, Nitschke M, Haddad R, et al. Production of Pseudomonas aeruglnosa LBI rhamnolipids following growth on Brazilian native oils[J]. Process Biochem, 2006,41 (2) : 483-488.
  • 8Thanomsub B, Pumeechockchai W, Limtrakul A, et al. Chemical structures and biological activities of rhamnolipids produced by Pseudomonas aeruginosa B189 isolated from milk factory waste [J]. Bioresour Technol, 2006,97(18) :1149-1153.
  • 9陈涛,杨世忠,牟伯中.微生物发酵液中脂肽类生物表面活性剂的测定[J].油田化学,2004,21(4):385-390. 被引量:14
  • 10吕应年,杨世忠,牟伯中.一种脂肽类生物表面活性剂产生菌的筛选[J].微生物学杂志,2005,25(2):4-8. 被引量:14

二级参考文献65

  • 1郝小燕,商立坚,郝小江.假鹰爪的黄酮成分研究[J].云南植物研究,1993,15(3):295-298. 被引量:10
  • 2赵玉瑞,周俊,王宪楷,黄晓琳,吴厚铭.千针万线草环肽A的结构修正[J].云南植物研究,1995,17(3):345-349. 被引量:8
  • 3赵玉瑞,周俊,王宪楷,黄晓琳,吴厚铭,谭宁华,陈昌祥.千针万线草环肽H的结构[J].云南植物研究,1995,17(4):463-467. 被引量:10
  • 4杨振宇,石梅,王大威,常剑飞,窦绪谋.大庆油田本源微生物群落分布及采油机理研究[J].石油学报,2006,27(B12):95-100. 被引量:24
  • 5Anita S K, Kalpana M, Bhavanath J. Evaluation of biosurfactant/ bioemulsifier production by a marine bacterium [ J]. Bull Environ Contain Toxicol, 2007, 79: 617-621.
  • 6Mohebali G, Ball A, Kaytash A, et al. Stabilization of water/gas oil emulsions by desulfurizing cells of Gordonia alkanivorans RIPI90A[J].Microbiol, 2007, 153: 1573-1581.
  • 7Kosaric N. Biosurfactants for soil bioremediation [ J]. Food Technol. Biotechnol, 2001,39 ( 4 ) : 295-304.
  • 8Jane Yii Wu , Kuei Ling Yell , WeiBin Lu ,et al. Rhamnolipid production with indigenous Pseudomonas aeruginosa EM1 isolated from oil-contaminated site [ J]. Bioresource Technology, 2008,99 : 1157-1164.
  • 9RoperM. M. The isolation and characterization of bacteria with the potential to degrade waxes that cause water repellency in sandy soils [ J ], Australian Journal of Soil Research,2004, 42 (4) :427-434.
  • 10Banat I M. Potentials for use of biosurfaetants in oil spills clean up and oil bioremediation [ J ]. Water Studies, 2000, 8:177-185.

共引文献70

同被引文献102

引证文献10

二级引证文献26

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部