摘要
目的:利用高效液相色谱法对天麻胶囊中天麻素的含量进行定量分析。方法:采用Kromasil C18(4.6 mm×200 mm,5μm)色谱柱,流动相为甲醇-水(55∶45),流速1.0 mL.min-1,检测波长220 nm,进样量10μL。结果表明天麻素浓度在8～80μg.mL-1范围内具有良好的线性关系(r=0.9995),天麻素平均回收率(n=6)为98.87%(RSD=1.4%)。结论:本法结果准确,操作简便,适合用于天麻胶囊的质量控制分析检验。
Objective：To develop an HPLC method for determination of gastrodin in Tianma capsules.Methods：Kromasil C18（4.6 mm×200 mm,5 μm） was adopted;The mobile phase was methanol-water（55∶45） at the flow rate of 1.0 mL·min-1;The detection wavelength was 220 nm and and the injection volume was 10 μL.Results：There was a good linear relationship with a range of 8-80 μg·mL-1 for gastrodin（r=0.9995）,the average recovery rate（n=6） was 98.87% with RSD of 1.4%.Conclusion：The method is simple,accurate,and can be used for the quantitative analysis of Tianma capsules.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2010年第1期152-154,共3页
Chinese Journal of Pharmaceutical Analysis
