摘要
目的:了解本院鲍曼不动杆菌中I类整合子的分布与基因盒结构,并探讨整合子与鲍曼不动杆菌耐药的关系。方法:收集鲍曼不动杆菌临床株,用纸片扩散法(K-B法)测定77株鲍曼不动杆菌对18种抗生素的敏感性,采用PCR法检测鲍曼不动杆菌Ⅰ类整合酶基因(intI1)并扩增其可变区,对PCR产物进行酶切分析,并测序分析整合子可变区携带的耐药基因盒。结果:鲍曼不动杆菌耐药现象十分严重。77株菌株中有49株含Ⅰ类整合子,阳性率63.64%,其中有45株(91.84%)整合酶阳性株扩增出可变区,共检测出4种耐药基因盒组合形式:aacA4(0.8kb)、dfrXII-orfF-aadA2(1.8kb)、aacA4-catB8-aadA1(2.3kb)、aacC1-orfX-orfX-orfX'-aadA1a(3.0kb)。整合子阳性菌株与整合子阴性菌株对多种抗生素耐药性存在差异。结论:Ⅰ类整合子与鲍曼不动杆菌耐药性密切相关,在多重耐药鲍曼不动杆菌耐药机制中起重要作用。
Objective：To study the distribution and structure of gene cassettes of class I integron in Acinetobacter baumannii in the First Affiliated Hospital of NJMU and explore the relationship between integron and its resistance.Methods：K-B tests were performed to detect the susceptibility of 77 isolates of Acinetobacter Baumannii randomly selected from the hospital to 18 kinds of antimicrobial agents,class 1 integrase gene（intI 1） and its variable region were amplificated by PCR,the products were analyzed by restriction endonucleases and sequenced.Results：77 isolates from different samples were serious antibiotic resistance.49 of 77（63.64%） isolates were found containing class 1 integron and 91.84%（45 / 49） of the intI 1 positive strains owned the variable region.Four different sizes（0.8 kb,1.8 kb,2.3 kb and 3.0 kb） of the variable region of integrons were amplified.Sequencing results identified four gene cassettes arrays：aacA4,dfrXII-orfF-aadA2,aacA4-catB8-aadA1 and aacC1-orfX-orfX-orfX＇-aadA1a.Significant association was demonstrated between integron carriage and resistance to antibiotics in Acinetobacter baumannii.Conclusion：class I integron is closely related to the resistance of Acinetobacter baumannii and plays an important role in its multi-drug resistance.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第1期82-86,共5页
Journal of Nanjing Medical University(Natural Sciences)
关键词
鲍曼不动杆菌
整合酶
整合子
基因盒
耐药性
Acinetobacter baumannii
integrase
integron
gene cassettes
resistance
