摘要
目的:观察不同剂量的天麻水溶性多糖(PGEB-3-H)对东莨菪碱所致记忆损伤模型小鼠的学习记忆是否具有改善作用。方法:行为学采用Morris水迷宫实验,测定小鼠脑组织乙酰胆碱(Ach)和丙二醛(MDA)含量,观察与模型组的差异。结果:与模型组(MC)相比,PGEB-3-H低、中、高剂量组(100、200、400mg/kg bw.d)小鼠找到平台的时间(潜伏期)明显缩短,且高剂量组(400mg/kg bw.d)与MC组之间差异极显著(P<0.01);高剂量组(400mg/kg bw.d)小鼠脑组织Ach含量显著高于生理盐水组(NC)和阳性对照组(PC);中等剂量组(200mg/kg.d)小鼠脑组织MDA含量显著降低。结论:PGEB-3-H能改善东莨菪碱所致的记忆损伤小鼠学习记忆能力,其作用机理可能是通过提高脑组织Ach含量。
Objective： To investigate the effect of PGEB-3-H,a soluble polysaccharide from Gastrodia elata Blume,on scopolamine-induced learning and memory disorders in mice.Methods： Learning and memory ability in mice were evaluated by Morris water maze.Acetylcholine and malondialdehyde（MDA） content were measured in brain tissues of mice.Results： Compared with model group,the time for passing water maze of mice treated with PGEB-3-H at doses of 100,200 mg/kg bw.d and 400 mg/kg bw.d（namely,PGEB-3-H low-,medium-and high-dose groups,respectively） was obviously shorter in a dose-dependent mode,and there was an extremely significant difference between PGEB-3-H high-dose group and model group（P〈 0.01）.Moreover,PGEB-3-H high-dose group exhibited higher acetylcholine content in brain tissues when compared to normal saline group or positive control group.In addition,MDA content in brain tissues of PGEB-3-H medium-dose group exhibited a significant decrease（P〈 0.05）.Conclusion： PGEB-3-H can improve scopolamine-induced learning and memory disorders in mice,and its mechanism may be due to the increase of acetylcholine content in brain tissues.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第3期246-249,共4页
Food Science
基金
西南大学博士基金项目(SWU109005)
云南省省校省院科技合作支持项目(2005YX24)
关键词
天麻
多糖
东莨菪碱
学习记忆
MORRIS水迷宫
乙酰胆碱
Gastrodia elata Blume polysaccharide scopolamine learning and memory Morris water maze acetylcholine
