摘要
从对虾养殖池塘中筛选出多株具有好氧反硝化性能的芽孢杆菌，其中芽孢杆菌YX-6能够在14h内将P（亚硝酸盐氮）由10mg／L降至0；p（DO）为5．2—5．8mg／L时，该菌对亚硝酸盐氮的去除率接近100％．试验选取具有降氮功能的芽孢杆菌作为阳性对照菌，比较研究了芽孢杆菌YX-6在不同pH，温度，ρ（亚硝酸盐氮）和盐度时的好氧反硝化性能．结果表明，芽孢杆菌YX-6的好氧反硝化性能显著高于阳性对照菌（P〈0．05）．通过对芽孢杆菌YX-6的形态学观察、生理生化及16SrDNA分子鉴定，初步鉴定其为凝结芽孢杆菌（Bacilluscoagulans）．
Many Bacillus strains with aerobic denitrification character were isolated from the water and sediment of shrimp cuhure ponds. Among them,the Bacillus YX-6 could degrade nitrite nitrogen from 10 mg/L to zero in 14 h,as well as approximately 100% of nitrite nitrogen removal rate at a DO mass concentration of 5.2-5.8 mg/L. Furthermore, in comparison with the denitrifying Bacillus selected as the positive control, the denitriflcation characteristics of Bacillus YX-6 were tested under different pH, temperature, nitrite concentration and salinity. The results showed that the aerobic denitrification characteristics of Bacillus YX-6 were significantly higher than those of the positive control （P 〈 0.05 ）. According to morphological observation, physiological and biochemical properties, and 16S rDNA molecular identification, the Bacillus YX-6 was identified primarily as Bacillus coagulans.
出处
《环境科学研究》
EI
CAS
CSCD
北大核心
2010年第1期100-105,共6页
Research of Environmental Sciences
基金
国家公益性行业科研专项(nyhyzx07-042)
现代农业产业技术体系建设专项资金项目(nycyts-49-17)
