摘要
目的了解肺炎克雷伯菌老年患者分离株16SrRNA甲基化酶、氨基糖苷类修饰酶基因的存在状况。方法自2006年1～10月住院患者标本中分离并筛选出20株多药耐药肺炎克雷伯菌，微量肉汤稀释法检测20种抗菌药物的敏感性；PCR法检测16SrRNA甲基化酶、氨基糖苷类修饰酶基因。结果20株多药耐药肺炎克雷伯菌16SrRNA甲基化酶（rmtA、rmtB、rmtC、rmtD、armA和npmA）基因均为阴性；氨基糖苷类修饰酶aac（3）-Ⅱ基因阳性15株、aac（6’）-Ib基因阳性1株、ant（3”）-I基因阳性16株、aph（3’）-I基因阳性3株、ant（2”）-I基因阳性1株。结论研究结果显示，肺炎克雷伯菌老年患者分离株对氨基糖苷类药物耐药的主要原因是aac（3）-Ⅱ、aac（6’）-Ib、ant（3”）-I、aph（3’）-I和ant（2”）-I 5种氨基糖苷类修饰酶基因的存在；从肺炎克雷伯菌中检出aac（6’）-Ib-Cr型和aph（3’）-I均为国内首次。
OBJECTIVE To investigate the prevalence of genes encoding 16S rRNA methylase and aminoglycoside modifying enzyme（AME） in multi-resistant Klebsiella pneurnoniae from the old patients. METHODS The samples of 20 multi-resistant K. pneumoniae strains isolated were collected from inpatients from Jan to Oct 2006. The sensitivity of the isolates to 20 antibacterial agents was determined using a broth dilution method. The genes of 16S rRNA methylase and AME were analyzed hy polymerase chain reaction. RESULTS 16S rRNA methylase gene was identified in none of the 20 isolates. AME aac （3r）-lI gene was found in 15 isolates, aae （6~）- I b was identified in 1 isolate, ant （3＂）-I in 16 isolates, aph （a＇）-I in 3 isolates and ant （2＂）-I in 1 isolate. CONCLUSIONS The resistance to aminoglycoside of K. pneumoniae mainly due to the presence of AME aac（3）- II , aac（6＇）- I b, ant（3＂）- I , aph（3＇）- I and ant（2＂）- l in old patients, and the genes of aac（6r）- I b-Cr and aph （3r）- I in K. pneumoniae are firstly found and reported in China.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2010年第3期308-311,共4页
Chinese Journal of Nosocomiology
关键词
肺炎克雷伯菌
甲基化酶
氨基糖苷类修饰酶
耐药性
Klebsiella pneurnoniae
Methylase
Aminoglycoside modifying enzyme
Multi-drug resistance
