摘要
目的探讨胃癌患者血清血管内皮生长因子（VEGF）和转化生长因子β1（TGF—β1）的表达及二者联合检测的临床意义。方法采用酶联免疫吸附试验法（ELISA）定量检测84例胃癌患者和40例健康人血清VEGF和TGF—β1水平，并分析其与临床病理因素之间的关系。结果胃癌患者血清VEGF和TGF—β1水平明显高于健康人血清水平[（392．33±118．54）pg／mlVS（139．64±72．31）pg／ml，t=12．4098，P〈0．01；（692．8±14.5）mg／LVS（62．3±6.5）mg／L，t=262．3721，P〈0．01]，其水平与浸润深度、分化程度、有无转移、TNM分期有关（P〈0．01），与性别、年龄、肿瘤大小无统计学意义（P〉0．05），胃癌患者血清VEGF与TGF-β1水平呈显著正相关（r=0．475，P〈0．01）。结论血清VEGF和TGF-β1。水平与胃癌蛳曼润、转移密切相关，术前检测血清VEGF、TGF—β1水平对预测胃癌的侵袭和转移具有重要的临床意义。
Objective To investigate the serum levels of vascular endothelial growth factor （VEGF） and transforming growth factor-betal （ TGF-β1 ） and the clinical significance of combined detection of serum TGF-β1 and VEGF in patients with gastric carcinoma. Methods The serum levels of VEGF and TGF-β1 in 84 patients with gastric carcinoma and 40 healthy persons were measured with ELISA. The relationship among the expression of VEGF ,TGF-β1, and the clinical pathological parameters was analyzed. Results The serum levels of VEGF and TGF-β1 in patients with gastric carcinoma were significantly higher than that in healthy persons [ （392.33 ±118.54）pg/ml vs （139.64 ±72.31） pg,/ml, t = 12.4098,P 〈0.01; （692.8± 14.5） mg/L vs （62.3 ± 6.5 ）mg/L, t = 262. 3721,P 〈 0.01 ） ] , The expression of VEGF and TGF-[31 was significantly associated with infiltration depth, differentiation, metastasis, and TNM stage （P 〈0.01 ）, but age, gender, and tumor size had no correlation with the the expression of VEGF and TGF-β1 （ P 〉 0.05 ） ; There was obvious positive correlation between VEGF and TGF-β1 （ r = 0. 475, P 〈 0.05 ）. Conclusion VEGF and TGF-β1 serum levels are closely correlated to the invasion and metastasis of gastric carcinoma, combined detection on the levels of serum VEGF and TGF-β1 may be useful to preoperatively predict invasion and metastasis of gastric careinoma.
出处
《国际免疫学杂志》
CAS
北大核心
2010年第1期72-75,共4页
International Journal of Immunology
关键词
胃癌
转化生长因子Β1
血管内皮生长因子
Gastric carcinoma
Vascular endothelial growth factor
Transforming growth factor-betal
