摘要
原核生物差异基因筛选方面,目前代表性差异分析方法(RDA)、消减抑制杂交法(SSH)、限制性酶切片段差异显示(RFDD-PCR)以及cDNA-AFLP等技术经改良后,可用于原核生物差异基因筛选的研究。论文对这几种方法的优缺点及应用进行了介绍。
Representational difference analysis (RDA), suppression subtractive hybridization (SSH), cDNA amplified fragment length polymorphism (cDNA-AFLP), restriction fragment differential display PCR (RFDD-PCR) can be applied on screcning differential genes in prokaryotes. The basic principles,advantages and disadvantages of methods above were discussed and compared with those of their counterparts.
出处
《动物医学进展》
CSCD
北大核心
2010年第2期103-106,共4页
Progress In Veterinary Medicine