摘要
[Objective]The aim of this study was to explore the technical system of induced expression in vitro of goat mammary gland epithelial cell,and evaluate expression efficiency of mammary gland specific vector and foreign protein at the cell level.[Method]Goat mammary gland epithelial cell transfected by human lactoferrin gene was inducted by culturing in DMEM/F12 medium supplemented with 5 mg/L insulin,5 mg/L prolactin and 1 mg/L hydrocortisone.Supernatant was collected per 6 hours and concentrated.Expression situation of foreign protein were detected by SDS-PAGE and Western blotting.[Result]There was target protein expression in the induced culture medium,which molecular weight was about 42 kD.[Conclusion]The method used in this study can induce goat mammary gland epithelial cell to express foreign gene,it lays a foundation for researching heterologous expression of foreign gene and producing mammary gland bioreactor.
[客观]这研究的目的是在山羊乳腺的 vitro 探索导致的表示的技术系统上皮的房间，并且评估在房间的特定的向量和外国蛋白质铺平的乳腺的表达式效率[方法]山羊乳腺由人的 lactoferrin 基因的上皮的房间 transfected 被 culturing 在与 5 mg/L 胰岛素补充的 DMEM/F12 媒介征调， 5 mg/L 催乳激素和 1 mg/L hydrocortisone.Supernatant 每 6 个小时和 concentrated.Expression
基金
Supported by Doctoral Start Fund of Henan University of Science and Technology.
