摘要
分别采用低浓度玉米小斑病菌Hel minthospolium maydis T小种毒素(HMT毒素)和玉米小斑病菌T小种培养滤液中的提取蛋白及菌丝细胞壁的提取液,预处理不同玉米品种3叶期叶片后再接种高浓度HMT毒素,在无菌条件下培养5 d后测量叶片病斑大小,并测定玉米叶片内苯丙氨酸解氨酶(PAL)活性变化。结果表明:只有纯化的低浓度HMT毒素能够作为激发子诱导玉米获得系统性广谱抗性,但不同玉米品种所需的最适合浓度有差异;TC103以质量浓度0.025μg/mL毒素预处理效果最好,PAL活性最高;TB37和TMo17 2个自交系,以质量浓度分别为0.025μg/mL和0.050μg/mL的毒素预处理效果最好。
The maize leaves were pretreated respectively by low concentraion Helrninthospoliurn rnaydis T-toxin（HMT-T）, protein and cell-wall, which were extracted from cultivating filtrate at the stage of three-leaf, and then these leaves were treated by high concentration HMT-T and cultivated 5 d. The dimension of spots in infected leaves was calculated and phenylalanine ammonia lyase（PAL） activity determined. The result showed that only the HMT-T could act as elicitor in the cultivating filtrate. The suitable concentration of HMT-T was different for various varieties. As to TC103 the suitable concentration of toxin was 0. 025 μg/mL and the PAL activity of maize leaves reached its maximum degree in this case. The right concentration of TB37 and TMol7 were 0. 025μg/mL and 0. 050μg/mL respectively.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2010年第1期21-25,共5页
Journal of Huazhong Agricultural University
基金
科技部国际科技合作项目(2006DFB02480)
农业部948项目(2008-Z20)
河北省自然科学基金项目(C2006000744)
河北省科学技术研究与发展计划项目(07297162D)资助
关键词
玉米小斑病菌T小种
毒素
激发子
苯丙氨酸解氨酶
Helminthospoliurn maydis T （HMT）
toxin
elicitor
phenylalanine ammonia lyase （PAL）
