摘要
为了建立大蒜叶枯病抗性鉴定的方法,根据田间调查结果选取对大蒜叶枯病不同抗性的3个品种G087、G064、G039作为鉴别品种,对病菌离体接种鉴定中不同孢子悬浮液浓度、病菌接种部位、接种后不同培养温度、不同株龄接种,以及活体接种方法进行了比较和筛选。结果表明:采用4～5叶期大蒜的离体叶片,叶面或叶背接种,接种病菌孢子悬浮液浓度106个/mL,接种后置于21℃温度条件下培养7 d,统计病情指数和品种抗性类型,鉴定结果与品种的抗病水平一致;离体接种鉴定比活体接种法更简便、快速;离体和活体鉴定结果均与品种田间抗病性一致,可作为大蒜品种资源叶枯病抗性鉴定技术。
The purpose of the experiment is to establish an evaluation method of garlic resistance to tip blight. According to the results of field investigation, the experiment collected 3 cultivars G087, G064,G039 with different disease resistance to garlic tip blight as the identification cultivars. The spore suspension concentration, the spore inoculation position and the culture temperature after inoculation and the plant age for in vitro identification and the methods for in vivo identification have been compared and screened. The investigating disease index and the cultivar resistant types were measured and the best in vitro identification results which was in agreement with the resistance level of the identification cultivars were gotten under the conditions as follows, the inoculating leaves at 4-5 leaf stage of plant, inoculating on leaf face or leaf back, inoculating spores at the concentration of 10^6/mL, culturing the inoculated leaves at the temperature of 21℃ for 7 days. Both in vitro and in vivo identification results were in agreement with field disease resistance of the identification cultivars. However, the in vitro identification was simpler and faster than the in vivo identification. The established in vitro identification technology in this experiment is simple in method, short in identification cycle duration and accurate in result, which can be used to evaluate the disease resistance of garlic germplasm resources to tip blight.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2010年第1期26-30,共5页
Journal of Huazhong Agricultural University
基金
国家"十一五"科技支撑计划项目(2006BAD07B02)
国家公益性行业(农业)科研专项(200903018-7)资助
关键词
大蒜叶枯病
抗性
活体鉴定
离体鉴定
garlic tip blight
resistance
identification in vivo
identification in vitro
