摘要
通过倒置显微镜观察斜纹夜蛾细胞凋亡小体,采用MTT法测定细胞增殖抑制作用,并使用流式细胞仪检测细胞周期时相,研究了骆驼蓬碱、喜树碱、蓖麻碱、苦参碱、博落回碱和烟碱对斜纹夜蛾离体培养细胞系SL-1的作用方式和细胞毒理。结果表明:骆驼蓬碱5.0μg/mL、20.0μg/mL和喜树碱0.5μmol/L、1.0μmol/L处理后48～60 h,斜纹夜蛾离体培养细胞系SL-1表现出明显的细胞凋亡诱导作用,处理后12～48 h,明显抑制SL-1细胞增殖,流式细胞仪检测显示细胞阻滞于G2/M期;博落回碱和蓖麻碱5.0μg/mL、20.0μg/mL对SL-1具有细胞增殖抑制作用,以20.0μg/mL处理具有细胞周期时相阻滞作用,同样使细胞阻滞于G2/M期,但无明显的细胞凋亡诱导作用;烟碱和苦参碱在5.0μg/mL、20.0μg/mL对SL-1具有细胞增殖抑制作用。
In order to determine and contrast the mode of action and cell toxicity mechanism of six botanical alkaloids（namely, harmaline, camptothecin, ricinine, matrine, macleay cordata and nicotine） a- gainst insect cell line,the apoptotic bodies, the inhibition of cell proliferation and the cell cycle phases were investigated by inverted phase contrast microscope, MTT test, and flow eytometry, respectively. The results showed that obvious apoptotic bodies appeared in Spodoptera litura cultured cell line SL-1 after 48-60 h treatments with harmaline at concentrations of 5.0μg/mL and 20.0 μg/mL or camptothecin at concentrations of 0. 5 μmol/L and 1.0/μmol/L. 12-48 h after the same treatments, it showed obvious inhibition of cell proliferation and G2/M arrest of SL-1. It also showed that similar inhibitive effect on cell proliferation and G2/M arrest on SL-1 cell line after the treatments with macleay cordata alkaloid and ricinine at the concentrations of 5.0μg/mL and 20.0μg/mL respectively, but no obvious apoptosis induction. The only significant SL-1 cell line proliferation inhibition was got after the treatment with nicotine and matrine at the concentrations of 5.0μg/mL and 20.0μg/mL. These results provided some valuable information to the investigation of the diversity of activities of botanical alkaloids against insect pests and their cell toxicity mechanism.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2010年第1期31-36,共6页
Journal of Huazhong Agricultural University
基金
国家自然科学基金项目(30500335)
教育部全国优秀博士学位论文作者专项基金(2004061)资助
关键词
生物碱
斜纹夜蛾
细胞
botanical alkaloids
Spodoptera litura
cell
