摘要
目的:探讨QHF复方联合小剂量化疗药物顺铂(DDP)对小鼠H22肝癌血管生成的影响,观察其抑瘤效果和不良反应.方法:48只BALB/c小鼠右腋皮下注射小鼠H22肝癌细胞建立荷瘤模型,随机分设为QHF复方组、小剂量DDP、联合用药组(QHF+DDP)及生理盐水组(NS).以抑瘤率为指标,观察各药物对荷瘤小鼠肿瘤生长的抑制作用;以小鼠的一般状况及脾指数为指标,观察各药物对荷瘤小鼠的不良反应;光镜观察肿瘤组织形态;免疫组织化学方法检测各药物对小鼠肝癌组织中的微血管密度(MVD).结果:QHF组、DDP组和联合用药组的瘤质量均较NS对照组明显降低(0.63g±0.16g,0.45g±0.23g,0.33g±0.15gvs1.22g±0.22g,均P<0.01),联合用药组较QHF组显著降低(P<0.01).抑瘤率分别为47.45%、63.11%和72.95%.QHF组、DDP组和联合用药组小鼠移植瘤组织内的MVD数量较NS对照组明显降低(11.00±1.56,10.33±1.49,6.87±0.97vs19.93±1.02,均P<0.01);联合用药组与各单药组相比明显降低(均P<0.01).QHF与DDP联合应用组的不良反应较单用DDP组轻,生存质量较好.结论:QHF复方与小剂量DDP均具有抗肝癌血管生成的作用;QHF复方与小剂量DDP联合应用具有协同抗肝癌血管生成的作用,同时QHF复方具有提高生存质量和降低化疗药物不良反应的作用.
AIM：To investigate the effects of QHF formula in combination with low-dose cisplatin （DDP） on tumor angiogenesis and growth in H22 hepatocellular carcinoma in mice and observe their toxic reactions.METHODS：Forty-eight BALB/c mice were inoculated with mouse hepatocellular carcinoma H22 cells in the right axillary skin to establish a mouse model of H22 hepatocellular carcinoma.The model mice were randomly divided into four groups：QHF group,DDP group,QHF plus DDP group and normal saline （NS） group.The inhibitory effects of these drugs on tumor growth were evaluated by calculating the reduced rate of tumor growth.The toxicity of these drugs was examined by observing the general condition of mice and the spleen index.The morphological changes of tumor cells were observed under an optical microscope.The microvessel density （MVD） in tumor tissue was evaluated by immunohistochemistry.RESULTS：The tumor weight was significantly lower in the QHF group,DDP group,and QHF plus DDP group than in the NS group （0.63 g ± 0.16 g,0.45 g ± 0.23 g and 0.33 g ± 0.15 g vs 1.22 g ± 0.22 g,respectively;all P〈 0.01）,while the tumor weight was significantly higher in the QHF group than in the QHF plus DDP group （P〈 0.01）.The reduced rates of tumor growth in the QHF group,DDP group and QHF plus DDP group were 47.45%,63.11% and 72.95%,respectively.The MVD in tumor tissue was significantly lower in the QHF group,DDP group,and QHF plus DDP group than in the NS group （11.00 ± 1.56,10.33 ± 1.49 and 6.87 ± 0.97 vs 19.93 ± 1.02,respectively;all P〈 0.01）.Compared with the QHF group and DDP group,the MVD was significantly lower in the QHF plus DDP group （both P 〈0.01）.Furthermore,a lower incidence of toxic reactions and a better quality of survival were observed in the QHF plus DDP group than in the DDP group.CONCLUSION：Both of QHF and small-dose DDP have anti-angiogenic effects in H22 hepatocellular carcinoma in mice.QHF in combination with small-dose DDP has synergistic anti-angiogenic effects and can improve the quality of survival and reduce the incidence of toxic reactions in mice bearing H22 hepatocellular carcinoma.
出处
《世界华人消化杂志》
CAS
北大核心
2010年第2期113-118,共6页
World Chinese Journal of Digestology
基金
湖北省卫生厅中医药中西医结合科研基金资助项目
No.2005-455-33~~
关键词
QHF复方
顺铂
化学疗法
肝癌
血管生成
QHF Cisplatin Chemotherapy Hepatocellular carcinoma Angiogenesis
