摘要
目的研究模拟不同模式训练对大鼠血浆及肾组织血管紧张素Ⅱ及其受体-1的影响，并探讨其机制。方法（1）一次性力竭训练：大鼠随机分为对照组（A组），训练1组（B1组），训练2组（B2组），行一次性力竭跑台训练，检测训练结束（B1组）及24h后（B2组）尿液指标和血液、肾组织血管紧张素Ⅱ及其受体-1的改变。（2）负荷累积训练：大鼠随机分对照1组（C1组），中强度负荷累积训练组（D1组），对照2组（C2组），高强度负荷累积训练组（D2组），D1组行4周递增负荷跑台训练，D2组行8周递增负荷跑台训练，分别检测4周末C1组、D1组各项指标，和8周末C2组、D2组各项指标。结果（1）一次性力竭训练结束即刻B1组尿指标、血浆及肾组织血管紧张素Ⅱ、血管紧张素Ⅱ受体．1表达较A组明显增强（P〈0．05）。24h后B2组各指标较B1组有所下降，但仍高于A组（P〈0．05）。（2）负荷累积中强度训练4周后D1组尿指标、血浆及肾组织血管紧张素Ⅱ浓度较C1组明显升高，而肾组织血管紧张素Ⅱ受体1表达明显减弱（P〈0．05）。负荷累积高强度训练至8周，D2组尿指标、血管紧张素Ⅱ浓度及血管紧张素Ⅱ受体-1表达较C2组均明显升高（P〈0．05），肾组织血管紧张素Ⅱ浓度与尿白蛋白量的相关程度高于血浆血管紧张素Ⅱ。结论模拟不同模式、不同强度训练，可引起大鼠血浆及肾组织血管紧张素Ⅱ和肾脏血管紧张素Ⅱ受体-1表达发生不同的改变，并且与尿白蛋白量密切相关。
Objective To investigate effects of two different high intensity training modes on angiotensin Ⅱ （ AT Ⅱ） and ATIR in serum and kidney of rats, and also to investigate its pathogenesis. Methods Two different high intensity training modes were： the once-only exhausting training mode and the load accumulation training mode. （ 1 ） In the first training mode, rats were randomly divided into the control group （group A）, training group 1 （group B1 ） and training group 2 （group B2）. Animals in group B1 and B2 were made to take on the once-only exhausting running exercises on treadmill. Urine data and concentration of angiotensin Ⅱ in serum and kidney, and the expression of AT1R in kidney were determined with immunohistochemistry upon termination of training and 24 hours after training. （2） In the second training mode, rats were randomly divided into control group 1 （group C1 ）, the moderate intensity training group （group D1 ）, control group 2 （group C2） and the high intensity training group （group D2）. Rats in group D1 were forced to take on progressively moderate intensity running exercises on treadmill for 4 weeks. Rats in group 1）2 were forced to take on progressively high intensity running exercises on treadmill for 8 weeks. Various data of group C1 and D1 were determined at the end of the fourth week, and data of group C2 and D2 were also measured at the end of the eighth week. Results （ 1 ） In the exhausting training mode group, chemical data, such as urine protein, urease, concentration of angiotensin Ⅱ in serum and kidney, and the expression of AT1R in group B1 all ncreased significantly right after training （P 〈 0.05 ） , when compared with those of group A. Twenty - four hours later, all the data of group B2 decreased to some extent, when a comparison was made with those of group B1, but were still higher than those of the control group （P 〈 0. 05）. （2） In the load accumulation training mode, chemical data, such as urine protein, urease and concentration of angiotensin Ⅱ in serum and kidney elevated obviously in group D1 at week d, when compared with those of control group 1 （C1）, whereas the expression of AT1R decreased obviously （P 〈 0. 05）. When the high intensity training came to the end of week 8, all the data of group D2 were higher than those of group C2 （P 〈 0.05 ）. The relationship between the concentration of angiotensin Ⅱ in kidney and urine protein was obviously closer than that of angiotensin Ⅱ in serum. Conclusions Different training modes and intensity could induce changes to various degrees in concentration of angiotensin Ⅱ and the expression of ATIR, and might be positively correlated with urinary albumin. These effects might be of importance in pathogenesis of renal injury caused by high intensity training.
出处
《中华航海医学与高气压医学杂志》
CAS
CSCD
2009年第6期344-348,共5页
Chinese Journal of Nautical Medicine and Hyperbaric Medicine
基金
全军医药卫生科研基金课题（06MA015）
