期刊文献+

NYGGF4基因在人前体脂肪细胞分化过程中的表达及肿瘤坏死因子α对其调控的研究 被引量:4

Expression of the NYGGF4 gene during human preadipocyte differentiation and the regulative role of tumor necrosis factor-alpha
原文传递
导出
摘要 目的观察人前体脂肪细胞诱导分化过程中NYGGF4基因mRNA表达水平的变化,探讨重组肿瘤坏死因子α(tumor necrosis factorα,TNFα)对成熟脂肪细胞中NYGGF4基因表达水平的调节作用。方法体外培养人内脏来源的原代前体脂肪细胞(human preadipocytes—visceral,HPA—v),在诱导HPA-v分化成熟的基础上,应用不同浓度重组TNFα干预成熟脂肪细胞16h,或以10ng/mL TNFα分别干预不同时间,采用实时荧光定量逆转录PCR技术检测的NYGGF4 mRNA表达水平。结果(1)HPA—v诱导分化至第17d,具备成熟脂肪细胞特征;(2)NYGGF4基因低表达于人前体脂肪细胞中,随脂肪细胞分化成熟其表达水平逐渐升高;(3)随TNFα干预浓度增高及干预时间延长,人成熟脂肪细胞中NYGGF4 mRNA水平呈现逐渐升高的趋势。结论NYGGF4基因具随脂肪细胞分化成熟表达逐渐上调的特征,TNFα能显著上调成熟脂肪细胞中NYGGF4基因的表达,其效应具有剂量依赖和时间反应性。 Objective To observe the changes of NYGGF4 gene expression during human preadipocyte differentiation and to explore the regulative role of tumor necrosis factor α (TNFα) on NYGGF4 gene expression in matured human adipocytes. Methods Human preadipocytes-visceral (HPA- v) were cultured in vitro and differentiated into the matured adipocytes. Fully differentiated adipoeytes were treated with TNFα in different concentrations for 16 hours or at 10 ng/mL for various times. Levels of the NYGGF4 mRNA were evaluated by real-time reverse transcription-PCR(RT-PCR). Results (1) After induction of differentiation, more than 90% of the HPAs-v exhibited typical adipocyte morphology on the 17th day. (2) In human preadipocytes, the level of NYGGF4 mRNA expression remained low. The NYGGF4 mRNA level was gradually increased with the preadipocytes being differentiated into the matured adipocytes, and reached the highest level in the fully differentiated adipocytes. (3) Treatment of the human matured adipoeytes with TNFα resulted in a significant increase in the level of NYGGF4 mRNA. The upregulatory effect of TNFα on the NYGGF4 gene expression tended to be enhanced with the increasing concentrations and elongation of time. Conclusion During human preadipoeytes differentiation, the level of NYGGF4 gene mRNA expression increases gradually. TNFα could upregulate the expression of NYGGF4 mRNA in human matured adipocytes. The regulatory effect of TNFα on the NYGGF4 gene expression is of dose dependent and time correlated.
出处 《中华医学遗传学杂志》 CAS CSCD 北大核心 2010年第1期69-72,共4页 Chinese Journal of Medical Genetics
基金 国家自然科学基金(30571978,30973231) 江苏省医学领军人才项目(LJ200624)
关键词 NyGGF4基因 人前体脂肪细胞 细胞分化 肿瘤坏死因子Α NYGGF4 genes human preadipocyte cell differentiation tumor necrosis factor α
  • 相关文献

参考文献2

二级参考文献21

  • 1郭锡熔.人类肥胖病因的遗传学研究[J].实用儿科临床杂志,2006,21(11):647-648. 被引量:8
  • 2Qiu, J. Ni, Y. H. Gong, H. X. Fei, L. Pan, X. Q. Guo, M. Chen, R. H. Guo, X. R..Identification of differentially expressed genes in omental adipose tissues of obese patients by suppression subtractive hybridization[J].南京医科大学学报(自然科学版),2007,27(4):334-334. 被引量:34
  • 3Kopelman PG. Obesity as a medical problem. Nature 2000; 404: 635-43.
  • 4Spiegelman BM, Flier JS. Obesity and the regulation of energy balance. Cell 2001; 104: 531-43.
  • 5Visscher TL, SeidellJC. The public health impact of obesity. Annu Rev Public Health 2001; 22: 355-75.
  • 6Ogden CL, Carroll MD, Curtin LR, McDowell MA, Tabak CJ, Flegal KM. Prevalence of overweight and obesity in the United States, 1999-2004. JAMA 2006; 295:1549-55.
  • 7Samaras K, Kelly PJ, Chiano MN, Arden N, Spector TD, Campbell LV. Genes versus environment. The relationship between dietary fat and total and central abdominal fat. Diabetes Care 1998; 21: 2069-76.
  • 8Rankinen T, Zuberi A, Chagnon YC, Weisnagel SJ, Argyropoulos G, Walts B, et al. The human obesity gene map: the 2005 update. Obesity (Silver Spring) 2006; 14: 529-644.
  • 9Wang B, Zhang M, NiYH, Liu F, Fan HQ,Fei L, et al. Identification and characterization ofNYGGF4, a novel gene containing a phosphotyrosine-binding (PTB) domain that stimulates 3T3-L1 preadipocytes proliferation. Gene 2006; 379: 132-40.
  • 10Ceddia RB,Somwar R, Maida A, Fanq X, Bikopouls G, Sweeney G. Globular adiponectin increases GLUT4 translocation and glucose uptake but reduces glycogen synthesis in rat skeletal muscle cells. Diabetoloqia 2005; 48:132-9.

共引文献27

同被引文献68

引证文献4

二级引证文献24

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部