摘要
背景与目的研究表明表皮生长因子受体(epidermal growth factor receptor,EGFR)和环氧合酶-2(cy-clooxygenase-2,COX-2)在多种实体瘤中存在高表达,并且可以通过相应的信号通路调节肿瘤的生长、侵袭和转移。本研究旨在探讨EGFR和COX-2在人类肺癌组织中表达的生物学意义及相互之间的关系。方法应用组织芯片技术结合免疫组织化学SP法检测89例原发肺癌、12例淋巴结转移性肺癌、12例癌前病变(不典型腺瘤样增生)和10例正常肺组织中EGFR、COX-2蛋白的表达情况。结果EGFR在肺癌组、癌前病变组、淋巴结转移性肺癌组中的阳性表达率分别为59.6%(53/89)、41.7%(5/12)和66.7%(8/12),COX-2在上述三组中的阳性表达率分别为52.8%(47/89)、41.7%(5/12)和66.7%(8/12),均较正常组明显升高(P<0.05)。EGFR和COX-2的表达与肺癌的组织学类型、临床分期和淋巴结转移有关(P<0.05),而与组织学分级、性别、年龄无关(P>0.05)。COX-2的表达还与肺癌的大体类型有关(P<0.05)。EGFR和COX-2的表达呈正相关(P<0.01)。结论EGFR和COX-2在肺癌中的异常表达与肺癌的发生、发展和恶性程度有关,两者存在一定的协同作用。检测EGFR和COX-2的表达有助于肺癌的临床诊断和预后评估。
Background and objective Epidermal growth factor receptor （EGFR） and cyclooxygenase-2 （COX-2）,which can regulate growth,invasion and metastasis of tumor through relevant signaling pathway,have been detected in a variety of solid tumors.The aim of this study is to investigate the biological significance of EGFR and COX-2 expression in lung cancer and the relationship between them.Methods The expression of EGFR and COX-2 was detected in 89 primary lung cancer tissues,12 premaliganant lesions,12 lymph node metastases,and 10 normal lung tissues as the control by immunohistochemical method on a tissue microarray section.Results EGFR protein was detectable in 59.6%,41.7%,and 66.7% of primary lung cancer tissues,premalignant lesions and lymph node metastases,respectively; COX-2 protein was detectable in 52.8%,41.7%,and 66.7% of primary lung cancer tissues,premalignant lesions and lymph node metastases,respectively,which were significantly higher than those of the control （P0.05）.The positive ratios and the levels of the expression of EGFR and COX-2 proteins were closely related to histological type,clinical stage and lymph node metastasis of lung cancer （P0.05）,but not to histological grade,sex and age （P0.05）.COX-2 expression was related to gross type （P0.05）.A highly positive correlation was observed between EGFR and COX-2 expression （P0.01）.Conclusion Overexpression of EGFR and COX-2 may play an important role in the tumorgenesis,progression and malignancy of lung cancer.Detection of EGFR and COX-2 expression might be helpful to diagnosis and prognosis of lung cancer .
出处
《中国肺癌杂志》
CAS
2010年第2期107-111,共5页
Chinese Journal of Lung Cancer
关键词
肺肿瘤
EGFR
COX-2
Lung neoplasms
Epidermal growth factor receptor
Cyclooxygenase-2
