摘要
目的:探讨肝脏胞外基质Matrilin-2在肝再生中与卵圆细胞的关系及作用.方法:采用改良的Soft-Farber建立大鼠肝脏卵圆细胞增殖模型,对照组灌喂生理盐水.分别取术后2、4、6、9、12、15d大鼠肝组织,采用免疫组织化学以及Western blot的方法动态观察大鼠卵圆细胞增殖模型中肝脏胞外基质成分Matrilin-2的变化与卵圆细胞的关系.结果:肝脏部分切除术(partial hepatectomy,PH)后第2天,卵圆细胞开始向门静脉周围区域增殖,Matrilin-2主要出现在门静脉周围的肝窦状隙内;术后第9天,卵圆细胞进一步向肝实质内增殖,Matrilin-2表达增加;术后第12天,随着卵圆细胞分化为小肝细胞结节,大多数Matrilin-2位于结节周边,少数出现在结节内.Matrilin-2的含量自肝切除后第2天开始升高,第9天达到高峰,第12天后逐步恢复生理水平.结论:肝脏胞外基质成分Matrilin-2与卵圆细胞介导的肝脏再生存在紧密联系并发挥重要的调控作用.
AIM： To investigate possible involvement of extracellular matrix （ECM） protein matrilin-2 in oval cell-mediated liver regeneration in rats. METHODS： A rat model of hepatic oval cell proliferation was established using the modified Soft-Farber protocol. The control group was fed normal saline. On days 2, 4, 6, 9, 12 and 15 after partial hepatectomy （PH）, rat liver tissue samples were collected. The dynamic relationship between matrilin-2 protein expression and oval cell distribution during the proliferation and differentiation of oval ceils was analyzed using immunohistochernistry and Western blot. RESULTS： On day 2 after PH, oval cells began to proliferate around the portal area, and matrilin-2 deposition was observed in the hepatic sinusoids in the periportal area. On day 9, proliferating oval cells were present in the hepatic acini, and matrilin-2 upregulation was noted.
出处
《世界华人消化杂志》
CAS
北大核心
2010年第4期346-349,共4页
World Chinese Journal of Digestology
