摘要
目的通过检测乳腺癌癌旁组织中增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)、C-erbB-2、p53、雌激素受体(estrogen receptor,ER)?孕激素受体(progesterone receptor,PR)蛋白的表达,探讨适合中国女性保乳手术的安全切除范围。方法收集40例乳腺癌根治术患者的切除标本,将每一标本分为癌肿区和癌旁区,癌旁区再划分为癌旁1 cm、2 cm、3 cm及>3 cm组。采用免疫组化SP法检测以上5组乳腺组织中非典型增生的发生率以及PC-NA、c-erbB-2、p53、ER、PR蛋白表达的差异。结果距离原发癌肿越远,癌旁组织发生非典型增生以及c-erbB-2、p53蛋白阳性的比例越低(P<0.05);癌旁1 cm、2 cm组高危因素(非典型增生以及c-erbB-2、p53)的比例明显高于3 cm组(P<0.05),而癌旁1 cm与2 cm组间比较未见统计学差异(P>0.05);PCNA、ER、PR在癌旁组织中的表达与组织分化、淋巴结转移无明显关系(P>0.05)。结论在乳腺癌保乳手术中,推荐以癌旁2 cm作为安全切除范围。
Objective To detect the expressions of proliferating cell nuclear antigen（PCNA）,C-erbB-2,p53,estrogen receptor（ER） and progesterone receptor（PR） in order to explore the proper margin for breast conservative surgery on Chinese women.Methods We collected 40 resection specimens from breast cancer patients who had received radical surgery.Then we divided each specimen into primary tumor group and paracarcinoma group,the latter of which was further divided into 1cm,2cm,3cm and 3cm-plus groups.Incidence of epithelial atypical hyperplasia and gene expression characteristics of PCNA,c-erbB-2,p53,ER and PR were measured by pathological and immunohistochemical assay in the five groups.Results With the further distance from the primary tumor,the proportions of high-risk disease and positive gene expressions of PCNA,c-erbB-2 and p53 in paracarcinoma tissues gradually decreased（P〈0.05）.Higher risk and more positive expressions were related to paracarcinoma 1cm and 2cm groups than 3cm groups（P〈0.05）;no significant difference was found between paracarcinoma 1cm group and 2cm group（P〉0.05）.There was no correlation of PCNA,ER and PR expressions adjacent to breast cancer with tissue differentiation and lymph node metastasis（P〉0.05）.Conclusion Based on our research,we recommend that paracarcinoma 2cm is a safe and appropriater region for breast conservative surgery.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2010年第2期212-216,共5页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
陕西省科技攻关项目(No.2003k10-G36)~~
关键词
乳腺癌
保乳手术
非典型增生
增殖细胞核抗原
C-ERBB-2
P53
雌激素受体
孕激素受体
breast cancer
breast conservative surgery
atypical hyperplasia
proliferating cell nuclear antigen
c-erbB-2
p53
estrogen receptor
progesterone receptor
