摘要
目的:观察邻苯二甲酸二丁酯(DBP)对大鼠认知功能的影响。方法:15月成熟雄性Wistar大鼠40只,随机分为4组(n=10)、对照组(C组)、DBP暴露组(E组)、药物预防组(P组)、药物治疗组(T组)。E组食用经DBP浸泡后的食物3个月、P组食用经DBP浸泡后的食物3个月,期间每天注射抗小胶质细胞活化药物吲哚美辛(按照2.5mg/kg,2次/天,腹腔注射),T组食用经DBP浸泡后的食物3个月后使用吲哚美辛治疗(按照2.5mg/kg,2次/天,腹腔注射1周),N组给予同剂量的生理盐水腹腔注射。各组于停药后1d使用Morris水迷宫实验,末次水迷宫测试结束后1h处死大鼠,取海马组织进行化学比色法检测其海马组织内AChE活性。结果:E、P、T组与对照组组比较,大鼠学习记忆能力均明显降低(P<0.001),AChE活性明显增加(P<0.001);P、T组与E组,P组与T组相比,大鼠学习能力及记忆能力无显著性差异(P>0.05),AChE活性无显著性变化(P>0.05)。结论:DBP可引起正常大鼠认知功能障碍,其机制可能与大鼠海马组织AChE活性增加有关。
Objective：To observe the dibutyl phthalate（DBP）of newborn rat cognitive function.Methods：Forty 15-month-old mature male Wistar rats were divided into 4 groups（n=10）,the control group（C group）,DBP exposure group（E group）,drug prevention group（P group）,the drug treatment group（T group）.Group E by eating foods DBP after soaking 3 months,P group consumed by DBP food after soaking three months,during the daily injection of microglial cell activation anti-drug indomethacin（in accordance with the 2.5mg/kg,2 times/day,intraperitoneal injection）,T group consumed by DBP food after soaking three months after the use of indomethacin treatment（in accordance with the 2.5mg/kg,2 times/day,intraperitoneal injection of 1 week）,C group were given the same dose of DBP. In 1d after stopping the use of Morris water maze test,the last water maze test rats were killed after 1h,check for chemical colorimetry hippocampus detected AChE activity in hippocampus.Results：E,P,T group and blank group,the ability of learning and memory in rats were significantly lower（P〈0.001）,AChE activity increased significantly（P〈0.001）;P、T group and E group,P group and T group compared to rats learning ability and memory capacity of no significant difference（P〈0.05）,AChE activity was not significant（P〈0.05））. Conclusion：DBP can cause cognitive dysfunction in normal rats,the mechanism of rat hippocampus may be associated with an increase in the activity of AChE.
出处
《现代生物医学进展》
CAS
2009年第23期4447-4449,4466,共4页
Progress in Modern Biomedicine
基金
重庆市自然科学基金资助(CSTC2007BB7006)
