摘要
[目的]研究食品添加剂纳米氧化锌对人正常肝细胞HL-7702的毒性作用。[方法]采用不同浓度(5、10、25、50、100、250μg/ml)的纳米氧化锌对HL-7702细胞进行染毒;光学显微镜下观察细胞的形态学变化;采用MTT法检测纳米氧化锌对HL-7702细胞生长活性的影响;应用荧光探针活性氧检测技术检测细胞内活性氧(ROS)的生成情况。[结果]纳米氧化锌可引起HL-7702细胞形态变化;MTT检测显示,纳米氧化锌对HL-7702细胞的生长活性具有明显的抑制作用;荧光探针活性氧检测发现,染毒后HL-7702细胞内ROS的生成量增加。[结论]纳米氧化锌作为一种新型锌源,建议控制其添加量或使用量。
[Objective]The research aimed to study the potential biological toxicity of ZnO nanoparticles used as food additives on human liver cell lines HL-7702.[Method]Different concentrations（5,10,25,50,100,250μg/ml） of ZnO nanoparticles were exposed to the HL-7702 cell.Changes in cell morphology were observed by optical microscope.The cell activities were detected by MTT test.ROS production was detected by the increasing fluorescence of the oxidation-sensitive dye 2＇,7＇-dichlorofluorescein diacetate（DCFH-DA）.[Result]The results showed that ZnO nanoparticles led to changes in cell morphology.Cell activity was inhibited by ZnO nanoparticles significantly.And ZnO nanoparticles could lead to ROS production.[Conclusion]As a kind of zinc source,the amount of ZnO nanoparticles should be controlled when used in food.
出处
《安徽农业科学》
CAS
北大核心
2009年第36期18023-18024,18026,共3页
Journal of Anhui Agricultural Sciences
基金
国家高技术研究发展计划项目(2007AA100403)
