摘要
目的探讨巢蛋白在高胆固醇血症大鼠肾小管上皮细胞中的表达及其意义。方法用含4％胆固醇和1％胆酸钠的高脂饲料饲喂大鼠建立高脂模型，观察大鼠血脂、24h尿蛋白、血肌酐和肾小管问质病理改变，免疫组化法检测肾小管上皮细胞巢蛋白和α-平滑肌肌动蛋白表达。结果高脂大鼠血脂、尿蛋白和血肌酐升高，肾小管间质出现损伤和间质纤维化。随时间延长，肾小管上皮细胞逐渐出现巢蛋白和α-SMA表达。巢蛋白表达与总胆固醇、低密度脂蛋白、24h尿蛋白、血肌酐显正相关（r=0．963、0．830、0．944、0．706，P〈0．01），与肾小管间质损伤指数呈正相关（r=0．974，P〈0．01），与α-平滑肌肌动蛋白表达呈正相关（r=0．804，P〈0．01）。结论巢蛋白异常表达可能与高胆固醇血症肾间质纤维化和肾小管上皮细胞转分化密切相关。
Objective To explore the expression and significance of nestin in renal tubular epithelial cells in hypercholesterolemic rats. Methods Dietary-induced hyperlipidemia were induced in female SD rats by given 4% cholesterol and 1% cholic acid diet for 16 weeks. Changes of serum lipid, urinary albumin, serum creatinine and renal interstitial pathological changes were assessed. The expression of nestin and a-smooth muscle aetin （α-SMA） were detected by immunohistochemieal stain. Results The serum levels of total cholesterol, low density lipoprotein, urinary albumin and serum ereatinine were significantly increased in hyperlipidemia group, accompanied with renal interstitial injury and fibrosis. As time extend- ed, the expression of nestin and α-SMA in renal tubular epithelial cells were increased significantly. There was positive correlation among the expression of nestin and total cholesterol, low density lipoprotein, urinary albumin and serum creatinine （ r = 0. 963,0. 830,0. 944,0. 706, P 〈 0. 01 ）. Nestin also had a positive correlation with tubular-interstitial index（ r = 0. 974, P 〈 0. 01 ） and α-SMA （ r = 0. 804, P 〈 0. 01 ）. Conclusion The increased expression of nestin may be associated with renal tubular-interstitial fibrosis and tubular epithelial myofibroblast transdifferentiation in hypercholesterolemic rats.
出处
《中国医师杂志》
CAS
2010年第1期20-23,共4页
Journal of Chinese Physician
