摘要
目的探讨ERK-1与Cyclin D1在肝细胞肝癌组织中表达的临床意义、相关性及对预后的影响。方法应用免疫组织化学方法检测50例肝细胞肝癌组织、17例癌旁硬化肝组织及14例正常肝组织ERK-1与Cyclin D1的表达。结果1.ERK-1与Cyclin D1在肝细胞肝癌组织中的表达显著高于癌旁硬化肝组织和正常肝组织;2.ERK-1表达与临床分期、病理分级、年龄、肿瘤大小、癌栓等明显相关;3.Cyclin D1的表达与病理分级有关;4.ERK-1与Cyclin D1表达-～+者平均生存时间较短,生存率较低,预后较差;ERK-1与Cy-clin D1表达++～+++者平均生存时间较长,生存率较高,预后较好;5.在肝细胞肝癌组织中ERK-1与Cyclin D1的表达呈正相关。结论ERK-1、Cyclin D1的表达水平的升高在肝癌的发生发展中发挥协同、促进的作用,监测它们的表达能为肝癌早期诊断及评价肝癌患者的预后提供帮助。
Objective To identify the significance and effect on prognosis of ERK-1 and Cyclin D1 expression levels in hepatocellular carcinoma （HCC）. Methods Fifty specimens of HCC, 17 specimens of adjacent non-cancerous cirrhosis hepatic tissue and 14 specimens of normal hepatic tissue were fixed by 10% formaldehyde,embeded in paraffin after excision. ERK-1 and Cyclin D1 were detected with immunohistochemistry. Results 1. ERK-1 and Cyclin D1 expression frequency in HCC were significantly higher than that in adjacent non-cancerous hepatic tissue and in normal hepatic tissue. 2. ERK-1 expression obviously correlated with clinical stage, the pathological classification, age, the size of tumor and the portal vein tumor thrombosis. 3. Cyclin D1 expression only related with the pathological classification. 4. Both ERK-1 and Cyclin D1 negative or weak expression, the survival rate was lower and the prognosis was poorer. Both ERK-1 and Cyclin D1 moderate and strong expression, the survival rate was higher and the prognosis was better. 5. ERK-1 expression level was positively correlated with Cyclin D1 expression level in HCC. Conclusion Increased the expression of ERK-1 and Cyclin D1 played an important cooperative and stimulative role in hepatocellular carcinoma. Monitoring the expression of ERK-1 and Cyclin D1 greatly help early diagnosed the hepatocellular carcinoma and evaluated the patients’ prognosis.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2010年第2期175-180,共6页
Cancer Research on Prevention and Treatment
基金
河北省科技厅科技攻关计划资助项目(052761260)
