摘要
以NaOH作为沉淀剂,在水相中合成了直径约为10nm的Fe3O4磁性纳米粒子,以Fe3O4为核层原料以CdTe量子点为壳层原料合成了Fe3O4/CdTe磁性荧光复合粒子。合成的Fe3O4/CdTe磁性荧光复合粒子经测试具有良好的磁性核荧光性能。以Fe3O4/CdTe作为能量供体3,端修饰有淬灭剂BHQ-2的单链DNA作为能量受体合成分子灯塔探针,成功构建了荧光共振能量转移体系,所合成的探针可利用磁铁与游离的5,-DNA-3,-BHQ-2进行快速分离。该探针在和与探针DNA序列完全互补的目标弓形虫DNA杂交后,荧光强度得到了恢复,实现了对弓形虫DNA的高灵敏度检测。
Preparing Fe3O4 magnetic nanoparticles about 10nm in diameter by a coprecipitation method with NaOH as the precipitant. With Fe3O4 materials for nuclear layer and CdTe quantum dots as a shell synthesized Fe3O4/CdTe magnetic-fluorescent composite particles. The molecular beacon probe was synthesized with Fe3O4/CdTe as an energy donor and 3＇-DNA-BHQ-2 as an energy receptor,and successfully constructed a fluorescence resonance energy transfer system. The results showed that it can be separated the free 5＇-DNA-3＇-BHQ-2 with the magnet. After the probe hybridizated with the complementary Toxoplasma gondii DNA, the fluorescence intensity had been restored to achieve high sensitivity for detecting the Toxoplasma gondii DNA.
出处
《科技信息》
2010年第3期I0036-I0036,I0341,共2页
Science & Technology Information
关键词
分子灯塔探针
复合纳米粒子
荧光共振能量转移
磁性分离
Molecular beacon probe
composite nano-particles
fluorescence resonance energy transfer
magnetic separation
