摘要
应用固相萃取及高效液相色谱技术，建立了同时测定罗汉果及其提取物中甲基托布津和多菌灵残留的方法。罗汉果及其提取物样品经乙腈超声提取后，由弗罗里硅土固相萃取小柱净化，以Zorbaxextend—C18（150mm×4．6mm，5μm）色谱柱为分析柱，乙酸铵水溶液（含乙酸0．5％）-甲醇为流动相，线性梯度淋洗，紫外二极管矩阵检测器检测，波长为270nm。结果表明，甲基托布津和多菌灵在0．5—15．0mg·L-1范围内有良好的线性关系，方法的定量下限（S／N=10）分别为0．06、0．1mg·kg-1，加标回收率为77％～96％，相对标准偏差不大于7．1％。
A solid phase extraction （SPE） coupled to high performance liquid chromatographic （HPLC） method was developed for determination of thiophanate-methyl and carbendazim in momordi- ca grosvenori and its extract. The sample was extracted with acetonitrile by ultrasound-assisted extraction, and purified by florisil SPE column. The detection of thiophanate-methyl and carbendazim was carried out by HPLC using Zorbax extend-C18 （150 mm ×4. 6 mm, 5 μm） column as separation column, 10 mmol . L-1 ammonium acetate solution（ containing 0.5% acetic acid） -methanol as mobile phase. The UV detecting wavelength was set at ：270 nm. The result indicated that the calibration curves of thiophanate-methyl and carbendazim were linear in the range of 0.5 - 15.0 mg . L-1 with limits of quantification（LOQs） of 0. 06 and 0. 1 mg . kg-1, respectively. The spiked recoveries of thiophanate-methyl and carbendazim were in the range of 77% - 96% with RSDs （ n = 6） not more than 7.1%.
出处
《分析测试学报》
CAS
CSCD
北大核心
2010年第2期199-202,共4页
Journal of Instrumental Analysis
基金
广西区公益性科研院所基本科研业务费专项资助项目(2008AC205)
关键词
甲基托布津
多菌灵
罗汉果
高效液相色谱法
固相萃取
thiophanate-methyl
carbendazim
momordica grosvenori
high performance liquid chro- matography （ HPLC ）
solid phase extraction （SPE）
