摘要
研究对象是西南地区的成年人179名(汉族),其中专业运动员89人(举重23人,跳水29人,水球37人),优秀运动员(健将级)35人。无创提取受检者口腔黏膜基因组DNA,PCR扩增、电泳后,用凝胶成像系统分析ACTN3基因的多态性。结果:1.汉族普通人群组ACTN3基因型频率分别为:RR型0.3444、RX型0.5000、XX型0.1556,经Hardy-W e inberg平衡检验,达到遗传平衡(χ2=0.1232,P=0.7256),提示所选人群的资料对总人群具有代表性。2.运动员的ACTN3基因型频率分别为:举重运动员RR型0.2609、RX型0.6522、XX型0.087,跳水运动员RR型0.3448、RX型0.5517、XX型0.1034,水球运动员RR型0.4595、RX型0.324、XX型0.2162;差异具有统计意义(P<0.05)。优秀举重运动员中没有XX型,优秀跳水运动员中XX型占4.55%,而优秀水球运动员中XX型占11.11%,提示ACTN3蛋白的存在或缺乏与中国运动员不同运动技能的发展有一定关系。结论:ACTN3基因多态性分析对中国优秀运动员的选拔和培养有一定参考价值。
179 athletes of Han nationality including 89 professional ones and 35 talent ones are taken as the research subject. Their DNA is extracted with oral mucosa -swab kit. The ACTN3 polymorphism is analyzed with PCR. Results ： ACTN3 genotype frequency of Hart ordinary group is RR 34.44%, RX 50%, XX 15.56%. It is shown to be in Hardy - Weinberg equilibrium （ X2 = 0. 1232, P = 0. 7256）, which indicates that the selected group is representative. ACTN3 genotype frequency of weightlifters is RR 26.09%, RX 65.22%, and XX 8.7%. ACTN3 genotype fre- quency of divers is RR 34.48% , RX 55.17% , and XX 10.34%. ACTN3 genotype frequency of water polo players is RR 45.95% , RX 32.4% and XX 21.62% （P 〈0.05）. The XX genotype frequency of talent athletes in weightlifters, divers and water polo players is 0%, 4.55% and 11.11% respectively. It suggests that the presence or absence of ACTN3 is related to the development of different athletic skills. Conclusion： An analysis of ACTN3 polymorphism may be helpful for the selection and cultivation of Chinese talent athletes.
出处
《成都体育学院学报》
CSSCI
北大核心
2010年第2期80-83,88,共5页
Journal of Chengdu Sport University
基金
四川省科技厅科研基金资助(川科计2006J13-020)
