摘要
高效液相色谱法测定马比木中喜树碱的含量。色谱柱为PLATISILTMODS(4.6×250 mm,5μm);甲醇∶水(55∶45)为流动相;检测波长254 nm;柱温30℃;流速0.9 mL.mi n-1。喜树碱线性范围为0.2μg-1.8μg,平均回收率100.3%,RSD为2.0%。该方法分离效果好、专属性强、分析速度快、灵敏度高,可作为测定马比木中喜树碱含量的方法。
To determine the content of camptothecin in mappia foetida by HPLC. Take PLATISILTM ODS （4.6 × 150 mm,5μm） as the column, methanol and water（55：45） as the mobile phase. The inspect wavelengh is 254nm ; the column temperature is 30 and the velocity of flow is 0.9 mL · min - 1 ; the lineal range is 0.2 - 1. 8μg, the average rate of recover）, is100.3% and the RSD is 2.0%. This method, with the advantages of good separation effect, specificity, high sensibility, rapid analysis, can be used to determine the content of camptothecin in mappia foetida.
出处
《绵阳师范学院学报》
2010年第2期60-62,共3页
Journal of Mianyang Teachers＇ College
基金
绵阳师范学院科学研究项目(hx-09-10-4)
绵阳市科技局自然科学研究重点项目(08Y002-3)
关键词
HPLC
喜树碱
马比木
HPLC
campthothecin
mappia foetida
