摘要
目的:建立测定2种鬼臼毒素制剂中鬼臼毒素含量的高效液相色谱方法。方法:Dikma diamonsil C18色谱柱(4.6mm×150mm,5μm);柱温为25℃;流动相为水(A)-甲醇(B);流速1.0mL/min;梯度洗脱;检测波长为292nm。结果:鬼臼毒素在11.8-590μg/mL范围内线性关系良好(r=0.9999),疣迪搽剂和鬼臼毒素酊中鬼臼毒素的平均加样回收率分别为99.38%、99.62%。结论:文章中所建立的色谱方法快速,重现性、精密度及准确度均能满足疣迪搽剂和鬼臼毒素酊中鬼臼毒素含量测定要求。
Objective： To develop a HPLC method for determination of Podophyllotoxin in two preparations. Methods： HPLC was carried out, using a Dikma diamonsil Cj8 column （4.6mm×l50mm, 5μm） with a column temperature at 25℃, the methanol-water as mobile phase（using gradient） with flowing rate 1.0 mL/min. The detection wavelength was at 292 nm. Results： The method had good linear relationship within the range of 11.8-590 μg/mL for Podophyllotoxin, with the correlation coefficients 0.9999.The results of the average recovery of Youdi liniment and Podopbyllotoxin tincture were 99.38%, 99.62%. Conclusion： The rapid, accurate and reproducible RP-HPLC method was useful for quantification of Podophyllotoxin in Youdi Liniment and Podophyllotoxin Tincture.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2010年第3期426-427,共2页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
浙江省自然科学基金(No.Y2081030)~~
