摘要
采用台湾报道的白点综合征相关杆状病毒（WSBV）的一对特异引物；对中国对虾的杆状病毒进行PCR扩增，获得了预期的1447bp的扩增产物，将PCR产物用QIAEXII（玻璃奶）纯化；直接进行序列测定。部分序列测定及分析结果表明，中国对虾的杆状病毒的部分DNA序列与台湾WSBV的相关序列的同源性为98．7％，经计算机分析该段基因序列有6个ORF（开放读框）。该PCR引物可以用于中国对虾杆状病毒及其相关病毒的检测与比较研究。
A 1 447-bp DNA fragment from baculovirus of shrimp (Penaeus chinensis)was amplified using a primer pair of WSBV. The PCR product was purified by QIAEXII and sequenced. The partial DNA sequence data showed high (98. 7%) similarity with the seqence data of WSBV from GenBank. There were 6 ORFs in this DNA fragment. The PCR by this primer pair is an effective tool for the detection of, and comparative studies on,baculovirus of P.chinensis and other non-occluded baculovireuses.
出处
《海洋水产研究》
CSCD
1998年第2期5-9,共5页
Marine Fisheries Research
基金
863资助!8190404
