摘要
目的探讨乳腺癌组织中组蛋白脱乙酰基酶1(HDAC1)和DNA甲基转移酶1(DNMT1)的表达及临床意义。方法应用组织芯片和免疫组织化学SP法检测105例乳腺癌组织和37例癌旁组织中HDAC1和DNMT1蛋白的表达情况并分析其与ER、PR之间的关系。结果(1)HDAC1蛋白在105例乳腺癌中的阳性表达率为64.76%,在癌旁组织中的阳性表达率为35.14%,两组差异有统计学意义(P<0.01);DNMT1蛋白在乳腺癌中的阳性表达率为55.24%,在癌旁组织中的阳性表达率为24.32%,两组差异有统计学意义(P<0.01);(2)HDAC1和DNMT1蛋白的表达与患者年龄、瘤体大小、组织学类型、组织学分级、腋窝淋巴结转移、临床分期均无相关性(P>0.05);(3)乳腺癌组织中HDAC1和DNMT1蛋白在ER或PR阴性乳腺癌组织中的表达分别高于其在ER或PR阳性乳腺癌组织中的表达(P<0.01)。HDAC1与DNMT1蛋白表达具有一定的相关性。结论乳腺癌组织中HDAC1和DNMT1蛋白过表达与肿瘤发生发展密切相关。
Objective To investigate the expressions of HDAC1 protein and DNMT1 protein and their clinical significances in the breast carcinoma tissue. Methods The expressions of HDAC1 protein and DNMT1 protein were determined by tissue microarray and immunohistochemistry with SP system. Their correlations to estrogen receptor（ER） and progesterone receptor（PR） were analyzed. Results （ 1 ） The positive rate of HDAC1 protein was 64. 76% in breast carcinoma, which was 35.14% in noncancerous tissue group. There were significant differences between them（ P 〈 0. 01 ）. The positive rate of DNMT1 protein was 55.24% in breast carcinoma, which was 24. 32% in noncancerous tissue group. There were significant differences between them （P 〈 0. 01 ）. （2）The expressions of HDAC1 protein and DNMT1 protein were not related with the age, the tumor size, the histological element, the lymph node metastasis, and the clinical stage（P 〈 0.01 ）. （3） The expressions of HDAC1 and DNMT1 had negative correlation to ER and PR. The expression of HDAC1 was positively correlated to DNMT1. Conclusion The overexpressions of HDAC1 protein and DNMT1 protein closely correlated with the tumor carcinogenesis.
出处
《临床肿瘤学杂志》
CAS
2010年第2期101-105,共5页
Chinese Clinical Oncology
基金
2006年上海市科委科研计划资助项目(06DZ19505)
