摘要
从采集于云南大学的喜树中分离到了90株内生放线菌,经16SrRNA基因序列分析鉴定,分布于6个科10个属。对所有分离到的菌株进行抗菌活性检测,发现33.4%的菌株有抗菌活性。通过PCR方法,检测了PKSⅠ、PKSⅡ和NRPS基因,阳性检出率分别为31.1%,48.9%和45.6%。
Ninety endophytic actinomycetes were isolated from surface-sterilized samples of Camptotheca acuminata Decne. collected from Yunnan University. Results of 16S rRNA gene sequences analysis showed that they belong to 10 genera, 6 families. Among them, 33.4% of endophytic actinomycete cultures presented antimicrobial activity. The polyketide synthases I （PKS-I）, polyketide synthases II（PKS-II） and non-ribosomal peptide synthase （NRPS） sequences were respectively detected by PCR in 31.1%, 48.9% and 45.6% of tested strains.
出处
《微生物学通报》
CAS
CSCD
北大核心
2010年第2期211-216,共6页
Microbiology China
基金
NSFC-云南联合基金项目(No.U0932601)
