摘要
目的观察中介素1-53对大鼠肺缺血再灌注损伤(LIRI)肺组织过氧化物酶增殖物激活受体γ(PPAR-γ)的影响。方法将健康Wistar大鼠54只随机分为手术对照组(C组)、缺血再灌注组(IR组)、中介素干预组(D组)。每组分别在缺血45min、再灌注60min、120min3个时点处死6只大鼠,观察肺组织病理形态变化,观察各组肺湿干质量比值(W/D)、CINC-1含量、PPAR-γmRNA、PPAR-γ蛋白的改变。结果与IR组比较,中介素1-53预处理能够明显升高PPAR-γmRNA(P<0.05),减轻肺水肿,并降低CINC-1蛋白含量(P<0.05)。结论中介素1-53能上调PPAR-γ的表达,可能通过PPAR-γ的抗炎作用对LIRI起保护作用。
Objective To investigate the effect of intremedin 1-53 on peroxisome proliferator-activated reeeptor-γ（PPAR-γ） in rat lung ischemia-reperfusion injury. Methods Fifty-four healthy Wistar rats were randomly divided into three groups：surgical control group（C group）, ischemia-reperfusion group（IR group）and intermedin-pretreated group（D group）. At three time points（45 min ischemia, 60 rain and 120 min reperfusion）,6 rats from each group were killed,the lung histopathological changes were observed and the wet /dry weight ratio of lung tissue,CINC-1 content,PPAR-γmRNA and PPAR-3, protein in each group were determined. Results Compared with IR group,the pre-treatment by intermedin1-53 significandy increased PPAR-γmRNA（P〈0.05）,and decreased interstital edema and CINC-1content（P〈0.05）. Conclusion Intermedinl-53 may upregulate PPAR-γ expression,which has protective effects on rat lung ischemiareperfusion injury.
出处
《中国现代医生》
2010年第4期3-5,共3页
China Modern Doctor
