摘要
该研究测试了卡那霉素、头孢霉素、氨卞青霉素、潮霉素4种抗菌素对秀珍菇、平菇和杏鲍菇菌丝生长的影响。结果表明:卡那霉素、头孢霉素、氨苄青霉素对3种食用菌菌丝生长及菌落形态基本没有影响,有些浓度处理对菌丝生长还具有微弱的促进作用。潮霉素对3种食用菌菌丝生长有很大的抑制作用,12.5mg/L的潮霉素能够完全抑制秀珍菇的菌丝生长,50mg/L的潮霉素能够完全抑制平菇和杏鲍菇菌丝的生长,研究结果为进一步建立3种食用菌的农杆菌介导的遗传转化体系打下了基础。
The investigations were carried out that effect of four kind antibiotics,including kanamycin,ampicillin,ceftaxime and hygromycin on mycelia growth of three edible fungi Pleurotus species such as Pleurotus eryngii,Pleurotus ostreatus and Pleurotus geesteranus.The results showed that it were no significant influence that cefotaxime,kanamycin and ampicillin against three kind edible mushroom mycelial growth rate and clone morphology,moreover some treatment within the scope of experimental concentration can weakly promote the mycelial growth.Hygromycin strongly inhibited mycelial growth of three pleurotus species,and 12.5 mg/L of hygromycin can completely inhibit the growth of mycelia of P.geesteranus,and it need 50 mg/L hygromycin for achieving the aim of complete inhibition of the mycelia growth of P.eryngii and P.ostreatus.The results laid a foundation of further study on the construction of Agrobacterium Tumefaciens-mediated genetic transformation system of three Pleurotus species.
出处
《北方园艺》
CAS
北大核心
2010年第2期215-217,共3页
Northern Horticulture
基金
福建省教育厅资助项目(2007F5098)
漳州师范学院大学生创新资助项目(08xscxxsyxm19)
关键词
平菇
秀珍菇
杏鲍菇
抗菌素
菌丝生长速度
Pleurotus ostreatus
Pleurotus geesteranus
Pleurotus eryngi
antibiotics
mycelium growth rate
