摘要
目的:研究不同的低氧训练模式对心肌组织血管生成的作用,从微血管生成的变化和规律来探寻最佳的低氧训练模式。方法:将健康雄性SD大鼠60只,按体重随机分为6组,运动组采用10周递增负荷跑台运动训练,每周训练6天,运动量由第1周的速度为15 m/min、持续时间为25 min递增至第10周速度为28 m/min、持续时间为50 min,低练组每周二、四、六在相当于海拔1500 m的低氧环境中训练,一、三、五在常氧下训练。并且在低氧环境中居住,低氧环境由第1周相当于海拔1800 m递增至第10周相当于海拔3600 m。采用免疫组织化学、显微图象分析对心肌组织毛细血管密度、光密度水平、表达面积进行计数和检测。结果:CD34可较好标记心肌组织微血管,其中低氧训练组有大量的CD34蛋白表达。结论:低氧训练能显著增加心肌组织的血管生成,其中高住高练低练这一低氧训练模式对心肌组织微血管的生成效果最好。
Objective ： this paper studied the different hypoxic training pattern on angiogenesis of atrial tissue and from angiogenesis patten to serach for a best hypoxic training pattern. Method： 60 male SD rats were randomly divided into six groups. 10 weeks progressive treadmill exercise program were designed with intensity starts from 15m/min for 25rain at the first week to 28m/min for 50min in the last week and hypoxia degree from 1,800 m at the first week to 3,600 min the last week. It uses the immunity histochemistry and the micro image analysis to count and check the capillary density, opitical density level, expression area of atrial tissue. Results ： CD34 could preferably display microvessel in cardiac muscle. Amour of CD34 protein were detected on microvessel. Conclusion： Hypoxic training can significantly increase angiogenesis in myocardial tissue, and the living high exercising high - training low is the best model of hypoxic training.
出处
《山东体育学院学报》
CSSCI
北大核心
2010年第1期50-54,共5页
Journal of Shandong Sport University
基金
国家自然科学基金项目(30671011)
