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日本大白兔PRLR基因外显子10的克隆与测序

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摘要 采用聚合酶链式反应方法,从日本大白兔血液中提取DNA,扩增出泌乳素受体基因(PRLR)外显子10,并对其进行克隆与测序,获得长度分别为208 bp和224 bp的2段序列,经过拼接得到1段418 bp的序列。将序列提交到GenBank上,GenBank中的Blast分析表明,测序得到的日本大白兔PRLR基因与家兔的同源性为99%。通过与其他目动物比较,PRLR基因在不同目动物中同源性不高,而在同目动物中同源性很高,表明PRLR基因在同一目中具有较高的保守性,同时在进化过程中具有一定的物种特异性。
作者 杨洪雁 胡锐 王星 白秀娟
机构地区 东北农业大学动物科学技术学院 辽东学院农学院
出处 《江苏农业科学》 CSCD 北大核心 2010年第1期59-61,共3页 Jiangsu Agricultural Sciences
基金 辽宁省科技项目(编号:2005408002)
关键词 PRLR基因 克隆 测序 日本大白兔
分类号 S813 [农业科学—畜牧学]
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参考文献16

  • 1Jenkins Z A, Henry H M, Montgomery G M. Follistatin(FST) ,growth hormone receptor( GHR ) and prolaetin receptor ( PRLR ) genes map to the same region of sheep chromosome 16[J]. Animal Genetics, 2000,31:280.
  • 2Phillips I D, Anthony R V, Butler T G, et al. Hepatic prolactin receptor gene expression increases in the sheep fetus before birth and after cortisol in fusion [ J ]. Endocr Inology, 1997,138 (3) : 1351 - 1354.
  • 3Boutin J M, Jolicoeur C, Okamura H, et al. Cloning and expression of the rat prolactin receptor, a member of the growth hormone/prelactin receptor gene family [ J ]. Cell, 1988,53 : 69 - 77.
  • 4Lerant A, Kanyicska B, Freeman M E, et al. Prolactin (PRL) and its receptor: actions, signal transduction pathways and phenotypes observed in PRL receptor knockout mice [ J]. Endocr Rev,2001,17 (3) :262 -268.
  • 5Levi - Meyrueis C. High aminoacid variation in the intra - cellular domain of the pig prolactin receptor(PRLR) and its relation to ovulation rate and piglet survival traits [ J ]. J Anita Sci,2006,84 (8) :1991 - 1998.
  • 6Boutin J M, Edery M. Identification of a cDNA encoding along form of prolactin receptor in human hepatoma and breast cancer cells [J]. Mol Endocrinol, 1989,3 (9) : 1455 - 1461.
  • 7Gurmen D, Horseman N D. Molecular cloning, tissue distribution, and expression of the prolaetin receptor during various reproductive states in Meleagris gallotayo[ J]. Biol Reprod ,2001,65 (5) : 1231 - 1264.
  • 8Chen X,Horseman N D. Cloning,expression,and mutational analysis of the pigeon prolactin receptor [ J ]. Endocrinology, 1994,135:269 - 276.
  • 9Linzer D I,Jolicoeur C ,Edery M ,et al. Identification and sequence analysis of a second form of prolactin receptor by molecular cloning of complementary DNA from rabbit mammary gland [ J ]. Proc Natl Acad Sci,1989,86:2112 -2116.
  • 10Tanaka M,Maeda K,Okubo T. Double antenna structure of chicken prolactin receptor deduced from the cDNA sequence [ J ]. Biochem Biophys Res Commun, 1992,188 (2) :490 - 496.

二级参考文献41

  • 1曹果清,李步高,刘建华,杨文平.猪催乳素受体(PRLR)基因AluⅠ多态性与产仔数关系的研究[J].山西农业大学学报（自然科学版）,2004,24(3):253-255. 被引量：27
  • 2施启顺,柳小春,刘志伟,李小平,李永辉,唐凡.5个与猪产仔数相关基因的效应分析[J].遗传,2006,28(6):652-658. 被引量：69
  • 3BAZAN J F. Haemopoietic receptors and helical cytokines[J]. Immunol Today, 1990,11 (10) : 350-354.
  • 4SHIU R P,KELLY P A,FRIESEN H G. Radioreceptor assay for prolactin and other lactogenic hormones [J].Science Fertil, 1990,40(Suppl. ): 355-370.
  • 5HU Z Z,ZHUANG L, MENG J,et al. The human prolactin receptor gene structure and alternative pro moter utilization: the generic promoter Hp Ⅲ and a novel human promoter Hp (N)[J]. J Clin Endocrinol Metab ,1999,84(3):1 153-1 156.
  • 6ARDEN K C,BOUTIN J M,DJIANE J,et al. The receptors for prolactin and growth hormone arelocalized in the same region of human chromosome 5[J].Cytogenet Cell Genet, 1990,53(2-3):161-165.
  • 7MOODY D E,POMP D, BARENDSE W,et al. Assignment of the growth hormone receptor gene to bovine chromosome 20 using linkage analysis and somatic cell mapping[J].Animal Genetics, 1995,26 : 3 413.
  • 8BARKER C S,BEAR S,KELER T, et al. Activation of the prolactin receptor gene by promoter insertion in a moloney murine leukemia virus 2 induced rat thymorea [J]. Journal of Virology, 1992, 66 (11) 6 763-6 768.
  • 9ORMANDY C J,SUTHERLAND R L. At the cutting edge mechanisms of prolactin receptor regulation in mammary gland[J]. Molecular and Cellular Endocrinology, 1993,91 : C1-C6.
  • 10ROTHSCHILD M F, VINCENT A L, TUGGLE C K, et al. A mutation in the prolactin receptor gene is associated with increased litter size in pigs[J]. Animal Genetics,1998,29 (Suppl. 1) :69.

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江苏农业科学
2010年 第1期

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