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重组人粒细胞集落刺激因子对大鼠脑出血后神经功能损伤的保护 被引量：3

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摘要 目的探讨重组人粒细胞集落刺激因子(rhG-CSF)动员骨髓干细胞对大鼠脑出血(ICH)后神经功能损伤的影响。方法实验动物随机分为假手术组、治疗组、ICH组。利用鼠脑立体定位定向仪、采用尾动脉取自体血法制模型,治疗组于术后1h始腹部皮下注射G-CSF(50μg·kg-1·d-1),在相应时间点大鼠行神经功能评分(NSS)后处死,用免疫组化法观察脑出血周围组织caspase-3、Brdu蛋白表达。结果治疗组神经功能评分和caspase蛋白表达均明显低于ICH组,二者相比有显著差异(P<0.05),治疗组的Brdu阳性细胞均多于ICH组,但只有第1周的差别有显著性(P<0.05)。结论rhG-CSF动员骨髓干细胞对脑出血大鼠有明显的神经保护作用。
作者 贾丽君 范佳 刘群
机构地区 中国人民解放军第 吉林大学第二医院神经内科 吉林大学第一医院神经内科
出处 《中国老年学杂志》 CAS CSCD 北大核心 2010年第4期513-515,共3页 Chinese Journal of Gerontology
关键词 脑出血 重组人粒细胞集落刺激因子 骨髓干细胞 大鼠
分类号 R743.34 [医药卫生—神经病学与精神病学]
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同被引文献20

  • 1何晓英,付华,袁平,谭华,李小刚.粒细胞集落刺激因子对脑出血大鼠的治疗机制[J].中国老年学杂志,2014,34(9):2486-2489. 被引量：5
  • 2JOSHUA B. BEDERSON,LAWRENCE H. PITTS,MILES TSUJI,MERRY C. NISHIMURA,RICHARD L. DAVIS,HENRY BARTKOWSKI.Rat Middle Cerebral Artery Occlusion: Evaluation of the Model and Development of a Neurologic Examination[J].Stroke.1986(3)
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  • 4Sevimli S, Setrecker JK, Strazzer, et al. Endogenous brain prmec- tion by G-CSF after ischemic stroke [J]. Exp Neurol, 2009,217 (2) :472.
  • 5Zavala F, Abad S, Jones NC, et al. G CSFtherapy of ongoing ex perimental allergic encephalomyelitis via cytokine-based immune deviation [J]. Immunol,2003,154(3) : 1032.
  • 6Schabitz,cortis, Prior M J, et al. Neuroprotective effect of G CSF after cerebral isehemia [J]. Stoke, 2004,64: 533.
  • 7Mélissa S. Charles,Robert P. Ostrowski,Anatol Manaenko,Kamil Duris,John H. Zhang,Jiping Tang.Role of the pituitary–adrenal axis in granulocyte-colony stimulating factor-induced neuroprotection against hypoxia–ischemia in neonatal rats[J]. Neurobiology of Disease . 2012 (1)
  • 8Peter Pastuszko,Gregory J. Schears,Afsaneh Pirzadeh,Joanna Kubin,William J. Greeley,David F. Wilson,Anna Pastuszko.Effect of granulocyte-colony stimulating factor on expression of selected proteins involved in regulation of apoptosis in the brain of newborn piglets after cardiopulmonary bypass and deep hypothermic circulatory arrest[J]. The Journal of Thoracic and Cardiovascular Surgery . 2012 (6)
  • 9Marcelo P. Baldo,Ana P. C. Davel,Danilo M. Damas-Souza.The Antiapoptotic Effect of Granulocyte Colony-stimulating Factor Reduces Infarct Size and Prevents Heart Failure Development in Rats. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY . 2011
  • 10Grace Marcy B,Singh Vijay K,Rhee Juong G,Jackson William E,Kao Tzu-Cheg,Whitnall Mark H.5-AED enhances survival of irradiated mice in a G-CSF-dependent manner, stimulates innate immune cell function, reduces radiation-induced DNA damage and induces genes that modulate cell cycle progression and apoptosis. Journal of radiation research . 2012

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中国老年学杂志
2010年 第4期

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