摘要
目的观察尿样经控温油浴消化后尿碘结果的精确度和准确度。方法控温油浴代替电热消解仪消解尿样,采用"尿中碘的砷铈催化分光光度测定方法"(WS/T107-2006)测定尿碘。结果标准曲线线性范围为0～300μg/L,相关系数平均为-0.9998。精确度:测定尿碘为(67.6±10)、(110.5±10)、(223.4±10)μg/L的3份尿样,变异系数分别为3.1%、2.3%、4.7%(n=6)。准确度:测定碘含量为(78.4±9)、(150.5±10)μg/L的2份尿碘国家标准物质,相对标准偏差分别为4.8%和2.8%(n=6)。结论控温油浴可以取代电热消解仪消解尿样进行尿碘测定,具有控温精准,制作容易的优点。
Objective To determine the precision and accuracy of measurement of urinary iodine levels in urine samples digested by temperature-controlled oil bath. Methods The urinary iodine level in the urine samples, digested by temperature-controlled oil bath instead of electrothermal digestion devices, was determined according to the As-Ce catalytic spectrophotometry method for measurement of urinary iodine （ WS/T 107 - 2006 ）. Results The linear range of the standard curve was 0 - 300 μg/L, the average correlation coefficient - 0. 9998. In terms of the precision, the coefficients of variation in determining the urinary iodine levels of three samples with the contents of （67.6±10）, （110.5 ± 10）, （223.4± 10） μg/L were 3.1%, 2.3%, and 4.7% （n =6）, respectively. In terms of the accuracy, the relative standard deviations in determining the levels of two national standard substances of iodine-containing urine samples with the iodine content of （78.4±＋ 9） and （ 150.5 ± 10）μg/L were 4.8 % and 2.8 % （ n = 6）, respectively. Conclusion Temperature-controlled oil bath, featuring precise temperature control and ease of making, is a good substitute for electrothermal devices for the measurement of urinary iodine levels.
出处
《疾病监测》
CAS
2010年第1期72-73,78,共3页
Disease Surveillance
关键词
尿
碘
分光光度计
恒温水浴箱
控温油浴箱
urine
iodine
spectrophotometer
constant temperature water bath
temperature-controlled oil bath tank
